Calibration of Fluorescence Microscopes
A New Durable Multi-Dimensional Ruler
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We propose a new durable multi-dimensional ruler for the calibration and the alignment of fluorescence microscopes (wide-field, confocal, multiphoton), thanks to non-photo-bleaching fluorescent sub-micrometer patterns embedded in glass in three dimensions. This new and robust tool enables the measurement of stage repositioning, detector's features, illumination homogeneity, both lateral and axial resolutions as well as the spectral shape, intensity and lifetime response of the system.