Chapter 8: Cell Polarity - Focal Adhesion and Actin Dynamics in Migrating Cells
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This chapter is a hands-on tutorial where time lapse movies of migrating cells are analyzed with ImageJ and Matlab. You learn how to trace subcellular objects (here: focal adhesions) in presence of noisy backgound and how to extract features such as growth dynamics and shape for each object. Moreover you will be intoduced to optical flow methods to measure actin movement and you will discover relationships between actin network movement and focal adhesion dynamics.
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