Deep Dive in Multiphoton Imaging
Multiphoton Imaging Enters New Areas of Bio-Science
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In the past decade, Multiphoton Excited Fluorescence (MPEF) has firmly established itself as a major imaging technique for biological sciences. MPEF enables fast (video rate and beyond) 3D in vivo imaging of tissue and in some cases entire organisms.
The technique relies on high peak power femtosecond pulses generated in the red and near infrared (NIR), thus allowing deep penetration (>1 mm) and sustained continuous imaging over long periods of time by limiting issues such as sample photo-toxicity, heating and damage.
Julien Klein, Product Marketing
Philip G. Smith, Phd, Product Marketing
Spectra-Physics, a Newport Company
Santa Clara, CA, USA