Dual Inverted Selective Plane Illumination Microscopy (diSPIM)
ASI has developed a new form of light sheet microscopy with our collaborators. The system utilizes two water emersion objectives mounted at 45 degree angles above the sample on an inverted microscope, a third objective also can be used to view the sample from below. The top objectives are mounted on piezo positioning devices so precise image stacks can be obtained.
• Use conventional mounting/glass cover slips
• Generate 3D volumes with isotropic resolution (330 nm in all directions)
• Axial resolution is ~2x better than confocal or spinning disk systems
• Achieve a ~7-10 fold reduction in photobleaching
• Acquisition rates up to 200 images per second or 2-5 volumes per second (roughly 10x faster than spinning-disk microscopes)
Original publication in Nature Protocols: