Mar. 03, 2016
Applications

HyVolution

Super-Resolution Imaging with a Confocal Microscope

Since the invention of the microscope, there has been continual discussion about the possibility of showing more detailed features of specimens as compared to just magnifying them. Ernst Abbe was the first to explore the issue in depth and found there was a limit to the size of details visible in ordinary light microscopes [1].
 

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References
[1] Abbe EK: Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung, Archiv für Mikroskopische Anatomie 9 (1) 413–468 (1873)
[2] Lauterbach MA: Finding, defining and breaking the diffraction barrier in microscopy – a historical perspective, Nanoscopy 2012, 1:8
[3] Rayleigh (Strutt JW): On the theory of optical images, with special reference to the microscope, Philos Mag Ser 5 42(255): 167–195 (1879)
[4] Sparrow CM: On spectroscopic resolving power, Astrophysical Journal 44:76 (1916)
[5] Houston, WV: A compound interferometer for fine structure work, Physical Review, 29 (3), pp 478-484 (1927)
[6] Minsky M: Memoir on Inventing the Confocal Scanning Microscope, Scanning 10, 128-138 (2011)
[7] Wilson T: Optical Aspects of Confocal Microscopy, Chapter 3 in: Confocal Microscopy, Ed: Wilson T, Academic Press, London (1990)
[8] Borlinghaus RT: The white confocal, The European Physical Journal Plus. 127:131 (2012)
[9] Borlinghaus RT, Birk H and Schreiber F: Detectors for Sensitive Detection: HyD, in Current Microscopy Contributions to Advances in Science and Technology (A Méndez-Vilas, Ed) Formatex (2012)
[10] Parœmiologia Anglo-Latina, Clarke J (editor), London, 1639
[11] Scientific Volume Imaging: Image Restoration vs Image Enhancement https://svi.nl/ImageRestoration retrieved Jan 19th 2016
[12] Scientific Volume Imaging http://www.svi.nl/ExpectedResolutionImprovementImpFaq retrieved Nov 16th, 2015
[13] Hell SW, Dyba M and Jakobs S: Concepts for nanoscale resolution in fluorescence microscopy, Curr Opin Neurobiol, 14:599-609 (2004)
[14] Schrader M, Hell SW & van der Voort HTM: Potential of confocal microscopes to resolve in the 50-100 nm range, Appl Phys Lett 69 (24), pp 3644-3646 (1996)

 

Authors
Borlinghaus RT & Kappel C
Leica Microsystems Mannheim

Contact

Leica Microsystems GmbH
Am Friedensplatz 3
68165 Mannheim
Germany
Phone: +49 621 7028 2801
Telefax: +49 621 7028 8011

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