Sep. 21, 2017
Applications

Multiphoton Microscopy

A Satisfied Wish List

  • Lineage traced tumor cells are shown in magenta, blue, green, yellow, and red. All channels were recorded with two-photon excitation, using the SP8 DIVE by Leica Microsystems. Sample and image were kindly provided by J. van Rheenen, H. Snippert, Utrecht (the Nederlands,) and I. Steinmetz, Leica Microsystems Mannheim.Lineage traced tumor cells are shown in magenta, blue, green, yellow, and red. All channels were recorded with two-photon excitation, using the SP8 DIVE by Leica Microsystems. Sample and image were kindly provided by J. van Rheenen, H. Snippert, Utrecht (the Nederlands,) and I. Steinmetz, Leica Microsystems Mannheim.

The colorful picture shows colon tumor cells, fluorescently labeled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1. Lineage traced tumor cells are shown in magenta, blue, green, yellow, and red. All channels were recorded with two-photon excitation, using the SP8 DIVE by Leica Microsystems. Sample and image were kindly provided by J. van Rheenen, H. Snippert, Utrecht (the Nederlands,) and I. Steinmetz, Leica Microsystems Mannheim.

Read the complete article: here

References
[1] Göppert-Mayer M (1931) Über Elementarakte mit zwei Quantensprüngen. Göttinger Dissertationen.
Annalen der Physik 401/3: 273–294
[2] Sheppard CJR and Kompfner R (1978) Resonnant scanning optical microscope. Applied Optics 17/18 pp 2879-2882
[3] Helmchen F and Denk W: Deep tissue two-photon microscopy. Nature Methods 2/12 (2005) 932-940
[4] Borlinghaus RT and Kremer M: Laser Beam Shaping for Multicolor Multiphoton Microscopy. Leica Science Lab (2017)
[5] Gugel H, Neugart F and Böhm I: Scanning Microscope. World Pat WO2016/198694 A1 (2015)

Authors
R.T. Borlinghaus1 and B. Grießhaber1

Affiliation
1 Leica Microsystems CMS GmbH, Mannheim, Germany

Contact
Dr. Rolf T. Borlinghaus, Scientific Advisor
Leica Microsystems CMS GmbH
Mannheim, Germany
rolf.borlinghaus@leica-microsystems.com
www.leica-microsystems.com
 

Contact

Leica Microsystems GmbH
Am Friedensplatz 3
68165 Mannheim
Germany
Phone: +49 621 7028 2801
Telefax: +49 621 7028 8011

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