Apr. 17, 2015
Applications

Optical Slices - Two Ways

Confocal and Digital Light Sheet Imaging

  • Color coded three-dimensional image volume of eye specific GFP expression (3P3 promoter) in Drosophila melanogaster (Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz)Color coded three-dimensional image volume of eye specific GFP expression (3P3 promoter) in Drosophila melanogaster (Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz)

Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details, inaccessible to the naked eye. But it notoriously suffers from an annoying trouble: the limited depth of field. Our eye lens (an optical imaging instrument) features the same nuisance, but our brain smartly removes all notin-focus information before the signal reaches conscious cognition. This does not work, if the image is retained on a photograph.

By optimizing parameters, we can increase the depth of focus, but then we usually loose resolution and also the zscale (information on the z position).If it
was possible to record only the features in focus and measure the relative z position, we solve the depth of focus problem: upon recording a tight stack of images, we can then reconstruct the entire sample without the blurry shares and quantify objects in all three dimensions.

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Contact

Leica Microsystems GmbH
Am Friedensplatz 3
68165 Mannheim
Germany
Phone: +49 621 7028 2801
Telefax: +49 621 7028 8011

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