Optical Slices - Two Ways
Confocal and Digital Light Sheet Imaging
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Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details, inaccessible to the naked eye. But it notoriously suffers from an annoying trouble: the limited depth of field. Our eye lens (an optical imaging instrument) features the same nuisance, but our brain smartly removes all notin-focus information before the signal reaches conscious cognition. This does not work, if the image is retained on a photograph.
By optimizing parameters, we can increase the depth of focus, but then we usually loose resolution and also the zscale (information on the z position).If it
was possible to record only the features in focus and measure the relative z position, we solve the depth of focus problem: upon recording a tight stack of images, we can then reconstruct the entire sample without the blurry shares and quantify objects in all three dimensions.
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