Oct. 23, 2015
Applications

Quantitative In Vivo Imaging of Molecular Distances Using FLIM-FRET

Förster Resonance Energy Transfer (FRET) is a valuable tool for measuring distances on the nanometer scale. In this process, energy from an excited donor molecule is transferred to a nearby non-excited acceptor. As the energy transfer process is strongly distance dependent, the quenching of donor fluorescence can be directly correlated to the donor-acceptor distance. The article presented here provides a description of using FRET in combination with Fluorescence Lifetime imaging (FLIM) to map out molecular interaction between labeled fluorescent proteins. 

Read the full article in the PDF.

Register now!

The latest information directly via newsletter.

To prevent automated spam submissions leave this field empty.