Jul. 07, 2016
Applications

Simultaneous Multi-color, Multiphoton Fluorophore Excitation using Dual-color Fiber Lasers

  • TPEF fluorescence map excited simultanously at different wavelength emitted by the FemtoFiber dichro design bioMP. Both beams share the same beam path to guarantee a spatial overlap at the sample. By tuning the respective temporal delay of both beams to zero a virtual excitation at 890 nm takes place exciting also the GFP neutrophil cell as shown in the inset.TPEF fluorescence map excited simultanously at different wavelength emitted by the FemtoFiber dichro design bioMP. Both beams share the same beam path to guarantee a spatial overlap at the sample. By tuning the respective temporal delay of both beams to zero a virtual excitation at 890 nm takes place exciting also the GFP neutrophil cell as shown in the inset.

Advanced microscopy techniques provide crucial insights for a deeper understanding of biological and chemical processes. Due to its nonlinear excitation character, multiphoton imaging (e.g. TPEF, SHG, THG) enables a deeper penetration depth and higher spatial resolution than conventional techniques. Here we present a novel approach to two-photon excitation at various wavelengths, i.e. 2-color 2-photon excitation, simultaneously using Toptica’s FemtoFiber dichro design bioMP.

Read the complete article as PDF here.

Contact

Toptica Photonics AG
Lochhamer Schlag 19
82166 Gräfelfing, Bayern
Germany
Phone: 089/85837-0
Telefax: 089/85837-200

Register now!

The latest information directly via newsletter.

To prevent automated spam submissions leave this field empty.