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Storming the Barriers of Light

Innovation in 3D Imaging

Apr. 14, 2010
A1R MP multiphoton confocal system with Ti-E motorised inverted microscope.
A1R MP multiphoton confocal system with Ti-E motorised inverted microscope. more

Live cell imaging is one of the most rapidly evolving areas of life science research. The more researchers see, the more they want to see and this is driving innovation in imaging technologies to see deeper, clearer and faster. While light is the fundamental enabling factor in optical microscopy, light can also create significant barriers when trying to image biological samples. Visible light, for example, cannot pass easily through living tissue and is scattered, preventing it from reaching areas of interest in thicker specimens. In addition, the diffraction of light has always been considered the main factor limiting the resolution of conventional light microscopes (to ~300nm laterally and 500-800nm axially). Here we describe some exciting developments that help overcome these limitations and promise to expand imaging capabilities to previously unimagined levels.

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Keywords: A1R MP Light Microscopy Multiphoton Imaging N-SIM N-Storm Nikon Instruments

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