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True Surface Microscopy for Topographic Confocal Raman Imaging

Chemically Sensitive Surface Tracing

Feb. 07, 2012
Fig 1: 3D Confocal Raman image of a fluid inclusion in garnet (60x60x30 µm; Red: Garnet, Blue: Water, Green: Calcite, Turquoise: Mica)
Fig 1: 3D Confocal Raman image of a fluid inclusion in garnet (60x60x30 µm; Red: Garnet, Blue: ... more
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Confocal Raman imaging opened the door for many applications in Raman spectroscopy and imaging that were previously unavailable for measurement with conventional (non-confocal) Raman methods. However, high confocality always results in high focus sensitivity and this can make measurements difficult with rough and/or inclined samples. Especially when performing scans on a larger scale (scan size larger than 1 mm), this often necessitated careful alignment and extensive sample preparation. True Surface Microscopy adds optical profilometer functionality to a highly sensitive confocal Raman microscope. Using this profilometer function, topographic scans of several square millimeters can be acquired, similar to a very large AFM topography image. This large-area topography image can be used to trace the surface contours while acquiring a confocal Raman image.

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Keywords: Hyperspectral Imaging Material Analysis Raman Imaging Raman spectroscopy Topographic Scans WITec

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ging & Microscopy Issue 4 , 2012 as free epaper or pdf download

 

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