May. 13, 2013
Applications

Unmask the Hidden without Probes: CARS

Imaging of Delicate Samples with Coherent Anti-Stokes Raman Scattering Microscopy

  • Fig. 1: (left) Study of localization and grade of accumulation of lipids in different types of macrophages: Lipids are displayed in red, actin in green, 63x objective. Courtesy:  Alba Alfonso Garcia and Jeff Suhalim, Potma Lab, University of California, USA. Cells: Dr. Adelheid Kratzer, Department of Medicine Division of Pulmonary and Critial Care, University of Colorado.Fig. 1: (left) Study of localization and grade of accumulation of lipids in different types of macrophages: Lipids are displayed in red, actin in green, 63x objective. Courtesy: Alba Alfonso Garcia and Jeff Suhalim, Potma Lab, University of California, USA. Cells: Dr. Adelheid Kratzer, Department of Medicine Division of Pulmonary and Critial Care, University of Colorado.

Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically - which was associated with the term "magic bullet", the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis - though not specific enough not to cause substantial side effects. Screening many fluorescent dyes led to a long list of stainings which are used in histology, including dyes like DAPI or hematoxylin and eosin.

Authors
Dr. Rolf T. Borlinghaus
, Scientific Advisor
Dr. Stefanie Degenhartt, Scientific Advisor

Leica Microsystems CMS GmbH
Mannheim, Germany

Contact
Christine Ludwig (contact via email request button)
Leica Microsystems GmbH
Mannheim, Germany

Contact

Leica Microsystems GmbH
Am Friedensplatz 3
68165 Mannheim
Germany
Phone: +49 621 7028 2801
Telefax: +49 621 7028 8011

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