Photoswitchable Fluorescent Proteins for Nanoscopy

Photoswitchable Fluorescent Proteins for Nanoscopy. S. Jacobs and colleagues have recently presented several photoswitchable fluorescent proteins that are suitable for fluorescence nanoscopy techniques like photoactivation localisation microscopy (PALM). In the article by M. Andresen and co-workers (1), two different reversibly switchable fluorescent proteins bsDronpa and Padron with similar emissions but with opposing switching behaviours are described. A.C. Stiel and colleagues (2), developed two monomeric reversibly switchable red fluorescent proteins, rsCherry and rsCherryRev, again with antagonistic switching behaviours.

1) Nature Biotech. 26, 1035-1040; 2) Biophys. J. 95, 2989-2997

Keywords: Fluorescence nanoscopy Photoswitchable Proteins

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