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Webinar on Superresolution Widefield Microscopy from Leica Microsystems
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Using ground state depletion technology (GSDIM), it is possible to control the emission of fluorochromes and localize the position of single molecules down to a precision of 20nm.
This makes it possible to reconstruct fluorescent images with a resolution far beyond the diffraction limit.
True-to-detail imaging of the spatial arrangement of proteins and other biomolecules in cells and observing molecular structures - GSDIM makes this possible for researchers due to resolutions beyond the diffraction limit. The more insight science gains into these basic processes of life, the better it can find the causes of previously incurable diseases and develop suitable therapies.
One strength of GSDIM is that it uses conventional fluorescence markers to image proteins or other biomolecules within the cells with sharpness down to a 20 nanometers. This includes fluorophores, which are routinely used in biomedical work, such as fluorescent proteins and rhodamines.
With GSDIM, the fluorescent molecules in the specimen are almost completely switched off using laser light. However, individual molecules spontaneously return to the fluorescent state, while their neighbors remain non-illuminating. This way, the signals of individual molecules can be acquired sequentially using a highly sensitive camera system and their spatial position in the specimen can be measured and stored. Then, an extremely high-resolution image can be created from the position of many thousands of molecules. This enables cell components that are situated very closely to one another and cannot be resolved using conventional widefield fluorescence microscopy to be spatially separated and sharply reproduced in an image.
• Super-resolution widefield imaging
• Localization microscopy
• Theory of ground state depletion
• The Leica solution for Super-resolution imaging
• Application examples for GSD imaging
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Keywords: Depletion Widefield Microscopy Fluorochromes Ground State Depletion Microscopy GSD Imaging GSDIM high-resolution microscopy Leica Leica Microsystems Leica SR GSD Localization Microscopy Microscopy Resolution at the Molecular Level Super-Resolution Imaging Super-resolution Widefield Imaging Webinar
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