The new Diffusion Measurement module for Olympus ASW 2.1 software is specifically designed for use with the Olympus FluoView FV1000 confocal microscope system for live cell imaging. Intracellular diffusion is frequently analysed to assess the movements, interactions and microenvironments of intracellular molecules to determine the patterns and rates of molecular movement. In order to meet this need, the Olympus Diffusion Measurement Package provides users with the flexibility to undertake three different types of diffusion study: point fluorescence correlation spectroscopy; raster scan image correlation spectroscopy (RICS) and fluorescence recovery after photobleaching (FRAP). As a result, the Diffusion Measurement Package is ideal for a broad range of cell biology and biophysics applications.
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Keywords: FRAP image processing live cell imaging Olympus Point Fluorescence Correlation Spectroscopy RICS software
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