Carl Zeiss Introduces Redesigned Scale Objective Lens for 3D Imaging of Brain Tissue

Maximum intensity projection of an image stack through the intact brain of a 7 week old transgenic ... more

Carl Zeiss MicroImaging has announced the availability of the completely redesigned plan-apochromatic 20x/1.0 VIS-IR objective lens.

This lens makes it possible to acquire 3D images of nerve cells down to depths of 5.6 mm in intact tissue using a confocal laser scanning or multiphoton microscope, without having to section the brain. This permits the three-dimensional visualization of the branches of individual nerve cells and the imaging of their connections. In untreated tissue, the penetration depth now achievable is five to ten times more than that of a multiphoton microscope featuring traditional optics.

Using the microscope to visualize and track nerve cells deep into the brain is an important step in the decoding of the brain circuitry. The redesigned objective lens now brings this goal considerably closer to realization. Carl Zeiss has designed the plan-apochromatic 20x/1.0 VIS-IR objective lens for the clearing method known as Scale developed by Dr. Atsushi Miyawaki at the RIKEN Brain Science Institute in Japan. A special water-based reagent solution transforms the sheath substance of the nerve cells into a transparent matrix without impairing the signals from fluorescent marker and tracer substances.

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Keywords: 3D Atsushi Miyawaki Brain Mapping Brain Research Carl Zeiss Carl Zeiss Microscopy confocal laser scanning LENS Multiphoton Microscopy nerve cells Objective Lens RIKEN Brain Science Institute

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