Deep Imaging of Thick and Complex Biological Samples

The image shows a 3D representation of YFP-expressing neurons in an excised mouse hippocampus, ... more

With the SCALEVIEW 25x objective lens (NA 1.0) from Olympus, accurate 3D structural representations of brain tissue are not longer limited by the effects of tissue opacity and light scattering.

The objective has a super-long-working-distance of 4mm and works alongside Olympus's new SCALEVEW-A2 clearing agent, which renders samples nearly translucent while preserving fluorescent signals. Image quality, sharpness and brightness are then maximized as the objective is optimized to match the refractive index properties of the clearing agent. When used as part of a complete Olympus FluoView FV1000MPE multiphoton system, the new objective makes it possible to peer deeper into samples than ever before, allowing you to generate truly insightful results from intact specimens.

Related Articles :

• Objectives with Ultra-long Working Distances
• Ultra Stable Stages for Super-Resolution Microscopy
• Olympus Scaleview Multiphoton Microscope Objectives Wins Three Awards
• Olympus´s FluoView FV1200: Optimized for Live Cell Imaging
• Olympus Introduces Standalone Controller for the 5 Megapixel DP26 Camera

Keywords: 3D Imaging Brain Research FluoView FluoView FV1000MPE Light Microscopy Multiphoton Imaging Objective Objective Lens Olympus Olympus Europe SCALEVIEW tissue imaging

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