With the SCALEVIEW 25x objective lens (NA 1.0) from Olympus, accurate 3D structural representations of brain tissue are not longer limited by the effects of tissue opacity and light scattering.
The objective has a super-long-working-distance of 4mm and works alongside Olympus's new SCALEVEW-A2 clearing agent, which renders samples nearly translucent while preserving fluorescent signals. Image quality, sharpness and brightness are then maximized as the objective is optimized to match the refractive index properties of the clearing agent. When used as part of a complete Olympus FluoView FV1000MPE multiphoton system, the new objective makes it possible to peer deeper into samples than ever before, allowing you to generate truly insightful results from intact specimens.
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Imaging & Microscopy Issue 4 , 2012 as free epaper or pdf download