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Deep Imaging of Thick and Complex Biological Samples

Jan. 31, 2012
The image shows a 3D representation of YFP-expressing neurons in an excised mouse hippocampus, created using images captured with an Olympus FluoView FV1000MPE multiphoton microscope system using an XLPLN25XSVMP SCALEVIEW objective lens (image provided courtesy of Hiroshi Hama and colleagues at the RIKEN Brain Science Institute, Japan).
The image shows a 3D representation of YFP-expressing neurons in an excised mouse hippocampus, ... more

With the SCALEVIEW 25x objective lens (NA 1.0) from Olympus, accurate 3D structural representations of brain tissue are not longer limited by the effects of tissue opacity and light scattering.


The objective has a super-long-working-distance of 4mm and works alongside Olympus's new SCALEVEW-A2 clearing agent, which renders samples nearly translucent while preserving fluorescent signals. Image quality, sharpness and brightness are then maximized as the objective is optimized to match the refractive index properties of the clearing agent. When used as part of a complete Olympus FluoView FV1000MPE multiphoton system, the new objective makes it possible to peer deeper into samples than ever before, allowing you to generate truly insightful results from intact specimens.

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Keywords: 3D Imaging Brain Research FluoView FV1000MPE Light Microscopy Multiphoton Imaging Objective Objective Lens Olympus Olympus Europe SCALEVIEW tissue imaging

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