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Dual Wavelength Imaging – The Ultimate Solution

Hamamatsu Photonics’ ORCA-D2 for Dual Wavelength Imaging

Nov. 15, 2011
Hamamatsu Orca-D2
Hamamatsu Orca-D2 more

The ORCA-D2 CCD camera is designed around two ER-150 CCD devices.  It can capture simultaneous dual wavelength or multiple focal plane images.  Each CCD captures a field of view measuring 1280 (H) x 960 (V) pixels, and each CCD has independent exposure and gain settings to accommodate significantly different intensity levels between the two images as is often seen in FRET and ratio imaging applications.  

Image separation is accomplished with interchangeable optical blocks that incorporate beam splitters and emission filters. Selecting among the dichroic splitter optical blocks or a 50/50 optical block provides a simple and easy-to-use tool for a wide range of applications. By combining the optical blocks with the two high quantum efficiency ER-150 CCDs, it is easy to make dual images of wavelengths between 400 nm and 950 nm at frame rates up to 11 fps at full resolution and full 12-bit output.

It also features wide dynamic range; high sensitivity and low noise, courtesy of Hamamatsu's advanced CCD cooling technology; and Hamamatsu's proprietary vacuum-sealed chamber technology, which promotes long-term, maintenance-free operation with even the most demanding applications. The camera also features an IEEE1394b interface, 12-bit A/D converter, and various external trigger modes.

The ORCA-D2 is suitable for a variety of microscopy applications, including ratio imaging, single and dual wavelength fluorescence microscopy, FRET, dual wavelength TIRF microscopy and many more.

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Keywords: Camera CCD Camera Fluorescence Microscopy Fluorescence Resonance Energy Transfer FRET Hamamatsu Hamamatsu Photonics Orca D2 Ratio Imaging TIRF TIRF Microscopy

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