Four Colour Imaging

Colour Separation, Photo Stability and High Sensitivity

Mouse retinal tissue stained with DyLight 405 (pseudo-colored green)-donkey anti-rabbit IgG (JIR) ... more

   

Jackson Immuno Research Laboratories has recently added Dylight 405 to its range of conjugated secondary antibodies. Dylight 405-conjugated antibodies are excited maximally at about 400 nm and fluorescence with a peak at about 421 nm. They are very bright and photo stable, but their use is limited to confocal microscopes equipped with a 405 nm laser and appropriate emission filter. It is now possible to perform effective four color imaging with great colour separation, good photo stability and high sensitivity in both aqueous and permanent mounting media. The combination of Dylight 405, Dylight 488, Rhodamine Red-X and Dylight 649 maximizes colour separation.

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Keywords: Antibody confocal microscopy Imaging Jackson Immuno Research laser Life Science Light Microscopy

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