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A collaboration between Cobolt and Semrock, has resulted in the development of optical filters optimized for use with the Cobolt Mambo 594 nm orange laser in fluorescence instrumentation. To realize the benefits of this new laser wavelength, optical filters that are designed with matched spectral properties are needed. Semrock has announced a number of new filters designed specifically for instrumentation based on the 594 nm laser, and ideal for excitation and detection of Texas Red, Alexa Fluor 594, and the popular new fluorescent proteins mCherry and mKate. A new triple-band filter set for three-color microscopy, LF405/488/594-A, consists of an excitation filter, a beamsplitter, and an emission filter for simultaneous illumination with 405, 488, and 594 nm laser lines. Individual filters optimized for the orange laser are also now available, including a new laser-optimized dichroic beamsplitter, Di01-R594, with excellent edge steepness and precision optical flatness, and the new EdgeBasic BLP01-594R long-wave-pass filter designed for fluorescence imaging as well as Raman spectroscopy. These all hard-coated filters from Semrock offer the simplest route to achieving exceptional images with laser-based microscopes and other instruments.
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Keywords: 594 nm DPSS Laser BLP01-594R Cobolt Cobolt Mambo Di01-R594 EdgeBasic Fluorescence Imaging laser Laser Fluorescence Filters LF405/488/594-A optical filters Raman spectroscopy Semrock triple-band filter
Cobolt AB
Vretenvägen 13
17154 Solna
Sweden
Tel: +46 8 545 91232
Fax: +46 8 54591231
Web: http://www.cobolt.se
Semrock Inc.
3625 Buffalo Road
NY 14624 Rochester
Tel: +1 (0) 585/594-7000
Fax: +1 (0) 585/5943898
Web: http://www.semrock.com
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