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Launches for Bioimaging
ChromoTek developed RFP-Trap and GFP-Booster
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ChromoTek has launched the bioimaging tools RFP-Trap and GFP-Booster. RFP-Trap is used to identify and pull down interaction partners of proteins tagged with RFP (red fluorescent protein) and GFP-Booster restores or increases the GFP (green fluorescent protein) signal in super-resolution microscopy. RFP-Trap is a tool for biochemical and cell biological analysis of RFP-tagged fusion proteins in pull down assays (a frequently used in vitro method used to determine physical interaction between two or more proteins). Bulky molecules tend to disintegrate during the purification process in a pull down experiment and make it difficult to further analyze the proteins of interest by mass spectrometry or Western blot. The company found a way to circumvent this problem by using small recombinant binding molecules, so called nanotraps instead of antibodies. Nanotraps bind their targets with similar efficiency but are only a tenth of the size of conventional antibodies and much more stable. GFP is on the other side to study protein localization and dynamics in living cells. However, the photo stability and the quantum efficiency of GFP are not sufficient for super-resolution microscopy (e.g. 3D-SIM or STED) of fixed samples from cells expressing GFP fusion proteins to visualize specific structures. The company developed a GFP booster consisting of a chemical fluorescent dye that is covalently attached to a GFP Nanotrap thus providing high fluorescence intensities and signal-to-noise ratios.
Keywords: 3D-SIM Bioimaging ChromoTek GFP-Booster green fluorescent protein Nanotrap Red Fluorescent Protein RFP-Trap STED Super Resolution Microscopy
ChromoTek GmbH
Großhaderner Str. 2
82152 Planegg , Bayer
Germany
Tel: +49 89 23526927
Fax: +49 89 218074236
Web: http://www.chromotek.com
Imaging & Microscopy Issue 4 as free epaper or pdf download
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