Leica Microsystems and APE, Berlin have developed a fully integrated confocal system with the capability for CARS (Coherent Anti-Stokes Raman Scattering) imaging, the Leica TCS CARS.
The microscope system is based on the Leica TCS SP5 II and benefits from imaging at video rate and a resolution of 8 x 8 Mpx for morphological studies.
A staining or markers are not necessary for imaging, since CARS signals are generated from the vibrational motion of molecules in specimens. The CARS technology is non-toxic and minimally invasive and perfectly meets the requirements of life scientists. The time and expense saved by the redundancy of specimen labeling makes the new Leica TCS CARS attractive to researchers in the areas of neuroscience, oncology, nutrition technology, pharmaceutics and many more.
The compact CARS laser device of Leica TCS CARS is fully controlled by the system software LAS AF. The specific beam routing ensures that two laser beams, provided by a pump laser and an optical parametric oscillator, temporally and spatially overlap to generate a specific CARS signal of molecules in the focal plane. Highly specific filter cubes support a large range of available wave numbers from 1200 cm-1 up to 3500 cm-1.
The selection of lasers for UV, IR and visible range covers the complete spectrum for confocal imaging and offers a great variety of applications for fixed specimens, living cells, tissue, and even whole animals.
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Keywords: APE CARS CARS microscopy coherent anti-Stokes Raman scattering Confocal Imaging confocal microscopy Label-free Imaging Leica Leica Microsystem Life Science live cell imaging Molecular Profiling Neuroscience Nutrition Technology Oncology Pharmaceutical Applications pharmaceutical industries TCSCompany Homepage
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Imaging & Microscopy Issue 4 , 2012 as free epaper or pdf download