New Opportunities for Live Cell Nanoscopy

  • The image shows an optically cleared rat kidney section. Nephrin immunolabelled with Star 635P. Excitation and STED with 640 nm and 775 nm, respectively. The sample was mounted in a fructose solution matching the refractive index of the immersion medium of the objective lens (RI = 1.46). Image dimension 83 µm x 83 µm, stack volume approximately 40 µm, colored coded 3D projection.  Sample courtesy of David Unnersjö-Jess, Department of Applied Physics, Royal Institute of Technology (KTH), Stockholm, SwedenThe image shows an optically cleared rat kidney section. Nephrin immunolabelled with Star 635P. Excitation and STED with 640 nm and 775 nm, respectively. The sample was mounted in a fructose solution matching the refractive index of the immersion medium of the objective lens (RI = 1.46). Image dimension 83 µm x 83 µm, stack volume approximately 40 µm, colored coded 3D projection. Sample courtesy of David Unnersjö-Jess, Department of Applied Physics, Royal Institute of Technology (KTH), Stockholm, Sweden

The STED WHITE objective Leica HC PL APO 93x/1.3 Glyc motCORR is a optic designed for Stimulated Emission Depletion (STED) imaging and resolves at the nanoscale.

Outstanding chromatic correction ensures optimal overlay of excitation and STED PSF in xy and z over the visible spectral range. Using the white light laser as excitation source for STED at 592/660/775, the objective allows the greatest choice of fluorophores ever for STED nanoscopy. A motorized correction collar allows precise and swift adjustment of the optical lenses to varying cover glass thickness and temperature changes. Deep imaging with 2D/3D STED is possible at room temperature or at 37°C.

Thick tissues mounted in glycerin-based embedding media or mounting media have a refractive index close to that of glycerin. Imaged with the STED WHITE glycerin objective, the refractive index is well matched. Its motorized correction collar allows adjusting for inhomogeneity and offer significantly improved image quality, outstanding 2D/3D STED performance and nanoscopy at unparalleled depth.

Contact

Leica Microsystems GmbH
Am Friedensplatz 3
68165 Mannheim
Germany
Phone: +49 621 7028 2801
Telefax: +49 621 7028 8011

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