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The Olympus XLSLPLN25XSVMP SCALEVIEW 25x objective lens has an 8 mm working distance, enabling the deep imaging of tissues, without the need for potentially damaging micro-dissections.
The objective has been optimized for use with the SCALEVIEW-A2 clearing reagent, which essentially clears bodily tissues of their opacity to provide a clearly defined image. By making the tissue transparent, while simultaneously minimizing light scattering, images can be obtained from 8 mm below the surface of the sample. Optimized for use with the Olympus FluoView FV1000MPE multiphoton microscope, users can obtain a complete system which avoids the occurrence of artefacts due to slicing, thus enabling you to be confident in the biological relevance of your findings.
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Keywords: 3D structure Brain FluoView FluoView FV1000MPE laser micro-dissection Multiphoton Microscopy neurobiology Neuroimaging Neurons Objective Objective Lens Olympus Olympus Europe Riken RIKEN Brain Science Institute SCALEVIEW tissue imaging
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Tel: +49 40 23773 0
Fax: +49 40 23773 973
Web: http://www.microscopy.olympus.eu
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Imaging & Microscopy Issue 4 , 2012 as free epaper or pdf download
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