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Polarization Extension for Confocal Laser Scanning Microscopes Allows Fluorescence Anisotropy Measurements
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Since several years, time-resolved fluorescence measurements can be performed with commercially available confocal laser scanning microscopes (LSM) that have been upgraded with a suited upgrade kit. The polarization extension for PicoQuant's upgrade kit for LSM can now also perform polarization measurements. The kit permits to access single molecule dynamics such as fluorescence anisotropy or polarization-resolved fluorescence correlation spectroscopy.
Field of Study
◦ Membrane Dynamics
◦ Molecular Rotation and Orientation
◦ Molecule Aggregation
◦ Cluster Formation
Calculation of the static anisotropy can be done using the PicoQuant's fluorescence lifetime imaging and correlation software SymPhoTime.
The polarization extension is directly mounted to the scan head of the confocal microscope. It splits the fluorescence according to its polarization and guides the light to two single photon sensitive detectors via two optical fibers. The insertion of the polarization sensitive beam splitter is reversible and switching between polarization or spectral light separation is easily possible.
Adaptable to the Microscopes
◦ Olympus FluoView FV300
◦ Olympus FluoView FV1000
◦ Zeiss LSM510
◦ Zeiss LSM 710
◦ Zeiss LSM 780
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Keywords: confocal laser scanning microscopy confocal microscopy Life Science Life Sciences molecular biology Olympus FluoView FV1000 Olympus FluoView FV300 PicoQuant SymPhoTime Time-resolved Fluorescence Time-Resolved Fluorescence Measurement Time-Resolved Microscopy Zeiss LSM 510 Zeiss LSM 710 Zeiss LSM 780
PicoQuant GmbH
Rudower Chaussee 29/IGZ
12489 Berlin, Berlin
Germany
Tel: +49 30 6392-6568
Fax: +49 30 6392-6561
Web: http://www.picoquant.com
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