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Extended DNA Dynamics Experiments by Applying Atomic Force Microscopy –
University of Nebraska selects JPK ForceRobot 300 System
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JPK Instruments has reported on the work from the Pharmaceutical Sciences Department of the Medical School at the University of Nebraska. The Department has selected the JPK ForceRobot 300 system to extend their studies applying atomic force microscopy in the measurement of single molecule force spectroscopy.
Aim of the Department
The goal of Professor Yuri Lyubchenko´s group is to unravel the role of the DNA dynamics at different levels as key mechanisms for various DNA functions including gene regulation, DNA recombination and the mismatch repair with the major focus on DNA recombination. The misfolding and aggregation of proteins are widespread phenomena leading to the development of numerous neurodegenerative disorders for which there is currently no cure.
Advantages of Using Atomic Force Microscopy
AFM helps to bridge the gap of various techniques which are capable of looking at the complex processes of protein misfolding and aggregation. However, it has the drawback of being very time and labor consuming to make measurements. This has been solved by the introduction of JPK's ForceRobot 300 system into the group.
Statement of Dr. A. Krasnoslobodtsev, Key Researcher
"AFM is my instrument of choice in my work towards a better understanding of these protein misfolding and aggregation phenomena. By measuring strength of interactions between protein molecules, it is possible to detect pathological misfolded conformations of proteins which are capable of triggering aggregation. Such conformations are characterized by elevated propensity to interact with one another. It is hoped that better understanding the mechanisms underlying self-assembly of proteins and peptides into nano-aggregates of various sizes and morphologies would eventually facilitate the development of efficient therapeutic and diagnostic tools for diseases associated with protein misfolding."
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Keywords: AFM Atomic force microscopy DNA DNA analysis instrument ForceRobot 300 Genomics JPK Instruments Life Science Protein Protein Aggregation protein structure Proteomis Scanning Probe Microscopy single molecule force spectroscopy
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