Imaging & Microscopy en Transmission Electron Microscopy: Chemical Reactions 'Filmed' at the Single-Molecule Level Scientists have succeeded in ‘filming’ inter-molecular chemical reactions – using the electron beam of a transmission electron microscope (TEM) as a stop-frame imaging tool (see video). They have also discovered that the electron beam can be simultaneously tuned to stimulate specific chemical reactions by using it as a source of energy as well as an imaging tool. Thu, 23 Mar 2017 08:38:00 +0000 Quantum Dots Illuminate Transport within the Cell Biophysicists from Utrecht University have developed a strategy for using light-emitting nanocrystals as a marker in living cells. By recording the movements of these quantum dots, they can clarify the structure and dynamics of the cytoskeleton. Their findings were published in Nature Communications. Wed, 22 Mar 2017 10:02:00 +0000 Read & Win: Modern Biophysical Chemistry - Detection and Analysis of Biomolecules The application of physical methods and principles to biological molecules has enabled the development of powerful bioanalytical techniques, in particular in the fields of microscopy and imaging. Mastery of these technologies usually requires a solid understanding of the underlying biological and physical principles, which is what this textbook is all about. Current standard methods used in research and development are explained here, including super-resolution microscopy and imaging techniques. Wed, 22 Mar 2017 09:45:00 +0000 Nanoparticles for Safe Imaging of Tumors Chemists from Russia and Switzerland created biosafe luminescent nanoparticles for imaging tumors and blood vessels damaged by heart attacks or strokes. The particles are made of hafnium oxide that can be used for intravenous injections, and doped with ions of rare earth metals. Scientists hope that their invention will become an alternative to the toxic quantum dots, and allow getting images of deep tissues without harming the human body. The study was published in Colloids and Surfaces B: Biointerfaces journal. Wed, 22 Mar 2017 08:37:00 +0000 Powerful, Multi-Wavelength LED Illumination for All Your Fluorophores X-Cite TURBO is a powerful, multi- LED light source for fluorescence imaging applications. Featuring Excelitas Technologies’ proprietary LaserLED Hybrid Drive, the TURBO provides maximum excitation power for all standard color wavelengths, including the ever-popular yet challenging yellow excitation band. Tue, 21 Mar 2017 07:22:00 +0000 Watching the Dynamics of the Orbital Angular Momentum of Plasmons A team of Israeli and German researchers from Technion in Haifa and the universities of Kaiserslautern, Duisburg-Essen and Stuttgart have been able to reveal for the first time the dynamics of the orbital angular momentum of plasmons. The researchers used a combination of extremely high-quality single crystals of gold, ultrafast laser pulses and an electron microscope. Their findings are published in Science. Tue, 21 Mar 2017 06:38:00 +0000 Imaging Individual Biomolecules on Surfaces Scanning tunneling microscopy produces high resolution images of surfaces and their adsorbates, ultimately reaching atomic resolution. Despite this impressive performance, the scope of this method is greatly limited by sample preparation. STM samples are prepared in ultrahigh vacuum, because they have to be chemically pure at the atomic level. Tue, 21 Mar 2017 05:53:00 +0000 Raman Imaging: World’s Oldest Fossils Unearthed Research led by Dr Dominic Papineau & Matt Dodd provide direct evidence for one of the oldest life forms on Earth - fossilized remains of microorganisms that are at least 3,770 million years old. Mon, 20 Mar 2017 12:15:00 +0000 UCL Winners at Wellcome Image Awards 2017 Images by UCL researchers are among the winners of the 2017 Wellcome Image Awards, given out annually to the most informative, striking and technically excellent images from healthcare and biomedical science. Mon, 20 Mar 2017 11:10:00 +0000 Making Microscopes from Droplets Liquid droplets are natural magnifiers. Look inside a single drop of water, and you are likely to see a reflection of the world around you, close up and distended as you’d see in a crystal ball. Researchers at MIT have now devised tiny “microlenses” from complex liquid droplets comparable in size to the width of a human hair. They report the advance in the journal Nature Communications. Mon, 20 Mar 2017 08:08:00 +0000 Event Driven Automated Microscopy Why Do Event Driven Microscopy? In a conventional micros­copy study the researcher defines an experimental configuration, typically a combination of time-lapse, z-stack, multi-channel, multi-position and multi-view settings. Importantly, everything the microscope will do is fully defined before the experiment is initiated. In an event driven approach sample variation is used to influence the experimental configuration. Sun, 19 Mar 2017 07:59:00 +0000 Redefining the Standard for Ultra Fast FLIM Imaging: RapidFLIM Fluorescence Lifetime Imaging (FLIM) is a versatile microscopy method, which combines fluorescence lifetime information with spatial localization, enabling the investigation of biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). Fri, 17 Mar 2017 13:25:00 +0000 Insights Tissue Clearing Today, imaging entire cleared samples is a vital necessity to understand biological systems and processes. Several protocols have been published describing different clearing protocols. The article will address some of the frequently used techniques. Thu, 16 Mar 2017 06:08:00 +0000 Imaging at the Speed of Light Tiny micro- and nanoscale structures within a material’s surface are invisible to the naked eye, but play a big role in determining a material’s physical, chemical, and biomedical properties. Over the past few years, Chunlei Guo and his research team at the University of Rochester have found ways to manipulate those structures by irradiating laser pulses to a material’s surface. Wed, 15 Mar 2017 08:24:00 +0000 AHFs´ PE-300ultra Allowes a Fast and Easy Controllable LED Illumination The illumination varies dramatically over time. So intensity from a conventional short-arc-lamp decreases through its life time. The lifetime of LEDs by far exceeds that of those lamps, and intensity remains broadly constant throughout its life providing stable and repeatable results. Sat, 11 Mar 2017 15:34:00 +0000 Scientists Characterize New Wonder Material Silicene Following in the footsteps of its famous cousin graphene, silicene, a two-dimensional material composed of silicon atoms, has fascinated researchers worldwide, in particular for its possible applications in the semiconductor industry. Bruno Grandidier, researcher at the IEMN in Lille France, reports on his recent work, investigating the spectral composition of this new "wonder material". Read the complete article here or click PDF button. Wed, 01 Mar 2017 09:11:00 +0000 Ultrafast Raman Spectroscopy Explained Bioimaging has enabled us to visualize biomolecules in action. However, current development of fluorescent reagents mainly relies on trial and error, because the rational design is hindered by limited mechanistic knowledge. The stimulated Raman technology, particularly on the ultrafast timescales, has provided new insights into bioimaging, but the operation of a sophisticated optical setup requires expertise. We present key application notes for implementation of tunable FSRS across disciplines. Wed, 01 Mar 2017 06:20:00 +0000 Toptica Presents Latest Laser Technology Toptica Photonics has introduced their latest laser innovations CTL, DLC DFB pro laser systems and low-noise frequency comb DFC. Tue, 28 Feb 2017 14:44:00 +0000 Olympus Wins IF Award for their Smart Microscope Design The Fluoview FV3000 and the CX23 microscope from Olympus have been recognised with the international iF award for exceptional product design for their workflow-oriented design quality across its range of life science microscopes. Tue, 28 Feb 2017 14:06:00 +0000 Tailor-Made Corrective Glasses Permit Unparalleled Concentration of X-Ray Beam An international team of scientists has tailored special X-ray glasses to concentrate the beam of an X-ray laser stronger than ever before. The individually produced corrective lens eliminates the inevitable defects of an X-ray optics stack almost completely and concentrates three quarters of the X-ray beam to a spot with 250 nanometres (millionths of a millimetre) diameter, closely approaching the theoretical limit. Tue, 28 Feb 2017 10:01:00 +0000 Hyperlens Devices: Spiky Nanostructures Capture Life’s Fine Details Optical microscopes that use lenses to bounce photons off objects have trouble distinguishing nanometer-scale objects smaller than the imaging beam’s wavelength, such as proteins and DNA. An innovative ‘hyperlens’ designed at A*STAR can overcome optical diffraction limits by capturing high-resolution information held by short-lived or evanescent waves lurking near a target’s surface. Thu, 23 Feb 2017 10:10:00 +0000 Park Systems Introduces Single Click Software SmartScan Park Systems has announced that its AFM operating software SmartScan is now available on Park XE series AFMs.  The software completely automatizes all of the functions of setting up and taking the image once done manually by the operator producing high quality nanoscale imaging in auto mode in five times the normal speed hence drastically boosts productivity single click reliable nanoscale images. Wed, 22 Feb 2017 10:07:00 +0000 Quantum Interpolation: High-Resolution Biomolecule Imaging Determining the exact configuration of proteins and other complex biological molecules is an important step toward understanding their functions, including how they bind with receptors in the body. But such imaging is difficult to do. It usually requires the molecules to be crystallized first so that X-ray diffraction techniques can be applied — and not all such molecules can be crystallized. Fri, 17 Feb 2017 09:59:00 +0000 Oxford Instruments Asylum Research Launches Its New Online European AFM Probe Store Oxford Instruments Asylum Research has launched its new European Atomic Force Microscopy (AFM) Probe Store. Thu, 16 Feb 2017 12:29:00 +0000 Shrinking Atomic Force Microscope to Dime-Sized Device Researchers at The University of Texas at Dallas have created an atomic force microscope on a chip, dramatically shrinking the size — and, hopefully, the price tag — of a high-tech device commonly used to characterize material properties. Thu, 16 Feb 2017 09:10:00 +0000