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Carbon Nanotube Hybridization with Ceramic Micro-Particles
Aug. 26, 2013

Carbon Nanotube Hybridization with Ceramic Micro-Particles

Owing to their unique anisotropic structure and their extraordinary properties, carbon nanotubes (CNTs) have spurred great interest in a wide range of application, and more particularly in materials science where the CNT hybridizations with other materials offer significant advantages. CNTs grown on alumina micro-beads and silicon carbide micro-platelets are among the most promising multi-scale combinations due to their outstanding thermal, electrical and mechanical properties. By simply adjusting the synthesis parameters, one can significantly affect the final structure and thus the properties of the hybrids, opening up a large area to explore. more
Stem Cell Imaging in Living Animals
Aug. 07, 2013

Stem Cell Imaging in Living Animals

Monitoring stem cells in their natural physiological environment is crucial to understanding stem cell ­differentiation and the generation of tissue. We applied high-resolution 3D multiphoton tomography in order to non-invasively visualize the stem cells of hair follicles. Single nestin GFP-expressing stem cells were tracked for up to five hours in living transgenic mice. The microenvironment was monitored by two-photon autofluorescence and second harmonic generation.
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Combined Holographic Optical Trapping and Imaging
Aug. 07, 2013

Combined Holographic Optical Trapping and Imaging

We combined holographic optical tweezers (HOT) with self-interference digital holographic microscopy (DHM). HOT allow flexible three-dimensional contactless moving of particles inside cells and positioning of bacteria on host cells while self-interference DHM provides simplified multi-focus quantitative phase imaging and 3D object tracking. Our results demonstrate novel ways for the analysis of the intracellular morphology and modeling of infection scenarios at single cell scale. more
Polarization and Nonlinear Microscopy
Aug. 06, 2013

Polarization and Nonlinear Microscopy

2-photon imaging of collagen-based biological tissues might benefit from the choice of particular incident polarization states. Here we further explore the effects of polarization on the visualization of ocular structures. Theoretical and experimental results suggest that linear polarization optimizes the imaging of samples containing mostly parallel elongated structures. However this type of polarization might not be the most appropriate when tissues present random distributed collagen patterns.
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Two Microscopes Integrated in One
Aug. 01, 2013

Two Microscopes Integrated in One

A high-NA light microscope can be retro-fitted onto a Scanning Electron Microscope giving the possibility to perform simultaneous high-resolution fluorescence and electron microscopy on the same area of a sample. more
In Situ Molecular Microscopy
Jul. 31, 2013

In Situ Molecular Microscopy

We present a novel approach for visualizing viral assemblies in liquid using transmission electron microscopy (TEM). We utilize a microfluidic chamber that fits within a TEM specimen holder and while inserted in the column, is completely isolated from the vacuum. This configuration proves suitable for imaging and reconstructing viral complexes in solution at 2.5-nanometer resolution. more
Nano-Mechanics on Skin Tissue
Jul. 29, 2013

Nano-Mechanics on Skin Tissue

Following traumatic injury, skin has the capacity to repair itself through complex biochemical changes. The dermis is remodeled over time affecting its mechanical behavior. AFM nanoindentation showed that the scar tissue exhibits stiffer behavior than healthy skin. Scar tissue shows weaker visco-elastic creep and reduced capability to dissipate energy at physiological frequencies than adjacent intact skin. AFM imaging showed a distinct orientation of collagenous fibrils in the scar tissue. more
Focused Ion Beam Ablation Tomography
Jul. 22, 2013

Focused Ion Beam Ablation Tomography

We explore the potential of a novel imaging method to exploit differential susceptibility of biological tissue to erosion by Focused Ion Beam (FIB) milling. The contents of a given cell (nucleus, Golgi apparatus, vacuoles) have different physical compositions and will ablate differentially under the ion beam. Sequential fine slices of frozen cells have been ablated, after which slice-by-slice image analysis of the extracted ablation vectors is back-converted to a FIB susceptibility parameter. more
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