You are here: HomeScience Overview › Archive

Science Archive

Thermal Imaging of Bacterial Cells
Mar. 14, 2014

Thermal Imaging of Bacterial Cells

Since its invention Atomic Force Microscopy has established itself as a versatile tool for imaging material systems & associated dynamic processes. In the early 90s the technique was further developed to incorporate the use of heated probes as means of scanning the sample surface. In this study E.coli bacterial cells were used in an attempt to not only further develop and optimize the use of thermal AFM, but also to try and gain further insight into their interactions with pharmaceutical materials. more
AFM-Based Force-Clamp Indentation
Feb. 25, 2014

AFM-Based Force-Clamp Indentation

The lipid bilayer rupture was here explored by means of AFM-based force clamp. For the first time to our knowledge, this technique has been used to evaluate how lipid membranes respond when compressed under an external constant force in the range of nN. We were able to directly quantify the kinetics of the membrane rupture event and the associated energy barriers, in distinction to the classic studies performed at constant velocity.
more
A Perfect Match: Choosing the Right Digital Camera for your Microscopy Application
Feb. 10, 2014

A Perfect Match: Choosing the Right Digital Camera for your Microscopy Application

Capturing and documenting a true-to-life representation of your sample is an important aspect of microscopy, achieved using the digital camera. Digital cameras specialized for microscopy come in all shapes and sizes, and selecting the best camera for your system can be a daunting task. It can be of help to consider four main features that essentially dictate camera performance: color reproduction, sensitivity, live image quality and resolution. Being aware of what these parameters really mean for your chosen application will guide you to a camera to complete your microscope system and truly enhance your experience. more
Exploring Nanoscale Viscoelastic Properties
Feb. 03, 2014

Exploring Nanoscale Viscoelastic Properties

Development of advanced materials relies on a detailed understanding of nanoscale morphology and mechanical properties. Atomic Force Microscopy (AFM) has become a key tool in material science by providing this information. Contact Resonance imaging has emerged as a powerful AFM technique for its ability to quantitatively characterize the viscoelastic response of materials, its applicability to a wide range of materials, and its ability to provide this information quickly and at high resolution.
more
Structure of the Diamond-lonsdaleite System
Jan. 13, 2014

Structure of the Diamond-lonsdaleite System

Hexagonal diamond or lonsdaleite (2H) is a new form of carbon which was found in meteorites [1]. For the first time it was obtained artificially [2] under conditions of static pressure exceeding 13 GPa and temperature greater than 1000 °C. Hexagonal diamond differs from the cubic one (3C) by the layers stacking. Crystal lattice of cubic diamond represent itself a sequence of atomic layers ABCABC..., whereas lonsdaleite lattice represents ABAB... stacking. Further, lonsdaleite was obtained mostly as a result of thermobaric treatment of carbon materials (for example, from graphite [3]). Britun V. et al. examined lonsdaleite obtained from graphite in high pressure chamber, and they have established that lonsdaleite is metastable martensite phase of carbon [4]. In [5] authors have synthesized nanocrystalline cubic diamond powder from C60, which contained lonsdaleite layers. Also diamond particularly was transformed into lonsdaleite during the diamond powder annealing [6].
more
Simple Processing in Fiji
Dec. 27, 2013

Simple Processing in Fiji

Microscopy has become an important element of the toolkit available to biologists. Advances in equipment and techniques have moved the microscope from a sole observational tool to a quantitative instrument. However, the requirements in terms of imaging in view of quantification, and image analysis may often seem daunting for non-specialists. In this communication, we propose a couple of simple and intuitive image analysis methodologies, evolving around median filtering, using functions in-built in Fiji [1]. The first application was to count labeled cells in mice tissue slices, which could be generalized to "object" counting in inhomogeneous samples. The second application was to quantify the signal to noise ratio (SNR) for each image of a z-stack, so that imaging parameters could be adapted to retain a constant SNR throughout the stack of images. more
What You Should Know about Image Noise
Dec. 09, 2013

What You Should Know about Image Noise

Insufficient light causes a snowstorm effect on many photos and videos, as a result of noise. It is important to know the different reasons for this phenomenon. The choice of the right camera for a particular application can help to eliminate the undesirable effect or to put it to good use. Pixel count, refresh rate and sensitivity are the decisive criteria for photographers when purchasing a camera. But they are also of interest to life scientists and users of microscopes. Unfortunately, there is another criterion that is often overlooked: the noise behavior of the camera or, to be more precise: its signal-to-noise ratio (SNR). Especially in professional applications, SNR is a major factor affecting image quality. Also, it is the characteristic that causes the greatest amount of misunderstanding.
more
Nanowires: A Cathodoluminescence Study
Nov. 28, 2013

Nanowires: A Cathodoluminescence Study

As features of modern electronics shrink, the demands of the spatial resolution of the characterization techniques increase. One family of potential building blocks for future devices is nanowires. We show how low-temperature cathodoluminescence can be used to study variations in the emission from two types of nanowires: Nanowires with a GaInAs segment in an otherwise GaAs core and radial InAs quantum wells on an InP core. more
RSS Newsletter


Follow Imaging & Microscopy on Twitter
.

Read Imaging & Micrsocopy Issue 2, 2015