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Light Sources for Epifluorescence Microscopy
Oct. 18, 2010

Light Sources for Epifluorescence Microscopy

If one could „bake" an ideal light source for widefield epifluorescence microscopy, the recipe would include many ingredients of the LED-based solutions which have recently hit the market: they are compact, long-lived and stable, they can be turned on and off in microseconds, their intensity is easily controlled and they are reasonably bright when large areas are to be illuminated under a microscope. Nevertheless, there are still many areas where arc lamps are a better choice. more
AFM-IR Enables Nanoscale Chemical Spectroscopy
Oct. 13, 2010

AFM-IR Enables Nanoscale Chemical Spectroscopy

Atomic force microscopy (AFM) has been enormously successful addressing problems in basic nanoscale research as well as applied problems in materials science and engineering. The technique as part of a family of scanning probe microscopy (SPM) variants has been widely credited with enabling the multi-billion dollar research investments in nanoscience and nanotechnology. However, a clear gap in AFM capabilities is the ability to chemically characterize regions of the sample. more
Architecture of the Crustacean Cuticle
Oct. 05, 2010

Architecture of the Crustacean Cuticle

Imaging of biomineralized matrices is challenging due to complex interconnections of organic and inorganic components forming their architecture. In this study histochemistry, fluorescence microscopy, TEM and AFM were used in combination to obtain related data on structure, composition and mechanical properties of extracellular calcified organic matrix constituting crustacean exoskeleton (fig. 1). more
Bridging Microscopes
Sep. 27, 2010

Bridging Microscopes

A comprehensive understanding of cellular function on the microscopic level in a whole cell or organism is the ultimate goal in system biology. Where system biology methods isolate individual molecular clusters for further investigations [1], microscopy offers the potential to directly "see" the complexes [2] ideally in their natural environment [3]. more
Tracking Cells in Microscopic Images
Sep. 23, 2010

Tracking Cells in Microscopic Images

Live-Cell Assays are used to study the dynamic functional cellular processes in High-Content Screening of drug discovery processes. The large amount of image data created during the screening requires automatic image-analysis procedures that can describe these dynamic processes. One class of tasks in this application is the tracking of cells and the description of the events and the changes in the cell characteristics, so that the desired information can be extracted from it based on data-mining and knowledge-discovery methods. more
Crystalline Calibration Standard for NPM
Sep. 20, 2010

Crystalline Calibration Standard for NPM

Investigations on nanopositioning and nanomeasuring machines (NPM) are aimed at measurement and manipulation of materials on a nanometer-scale. This requires an exact positioning and calibration of the used tools or Atomic Force Microscopy (AFM) tips and the possibility to retrieve a small area of interest (nm) on a large sample (µm - mm). A possibility to achieve a calibration on the atomic scale can be the use of well known lattice plane distances with high accuracy.

Nanopositioning and Nanomeasuring Machines
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HRSEM Study of Sn-Zn-Cu Film Formation
Sep. 13, 2010

HRSEM Study of Sn-Zn-Cu Film Formation

The formation of CuInSe2 and Cu2ZnSnSe4 thin films in the process of selenization precursor films was studied in use of combined microscopic (HRSEM) and spectroscopic (micro-XRF, EDS, micro-Raman and XRD) methods. more
Chitin
Sep. 06, 2010

Chitin

In this article we present a universal method for structural investigation of chitin by using correlative AFM-TEM microscopy. The advantage of this method is that polysaccharide chains, proteins, and biominerals can be analyzed simultaneously by AFM (protein-chitin fibril distribution) and analytical TEM (chemical composition of the same particular area).

Introduction
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