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3D Image Acquisition
May. 31, 2011

3D Image Acquisition

3D Image Acquisition: In fluorescence microscopy, various types of microscopes and digital imaging methods are increasingly used to image samples in three dimensions at highest possible resolution. This is technically achieved by layering images, which are taken at a fixed sample location but at different heights on top of each other. Improvements in Depth Resolution more
Restless Receptors - Dynamic Organization of Synapses
May. 30, 2011

Restless Receptors - Dynamic Organization of Synapses

Synapses are the switch-points in our brain for information transmission, learning and memory. And there is evidence to suggest that changes and malfunctions in synapses are partly responsible for a number of neurological and psychological disorders. Neuroscientists already know a lot about how signals are transmitted from neuron to neuron. Yet many synaptic processes are still not fully understood. Dr. more
Towards Real Time ESEM Imaging of Biological Processes
May. 26, 2011

Towards Real Time ESEM Imaging of Biological Processes

Historically, electron microscopy of dynamic biological processes has been impossible to achieve in real time because conventional electron microscopy requires specimen fixation, dehydration and metallic coating. The advent of the Environmental Scanning Electron Microscope (ESEM) removed these restrictions, allowing fully hydrated samples to be imaged in their native state. This raises the possibility of secondary electron imaging of dynamic biological processes. Well Suited to Biological Imaging more
Tensile Testing of Microstructures
May. 13, 2011

Tensile Testing of Microstructures

The small size of micro- and nano-structures makes tensile testing challenging. In this study we meet this challenge by combined use of a Focussed Ion Beam (FIB) in Dual Beam configuration, an AFM-cantilever, and a micromanipulator which provide the required accuracy and versatility to measure the mechanical properties of nanowires by tensile testing. AFM cantilevers with a big range of force constants principally enable us to measure the tensile behavior of a great variety of materials. more
Hyperbaric Atomic Force Microscopy
May. 12, 2011

Hyperbaric Atomic Force Microscopy

We report on the development of a Hyperbaric Atomic Force Microscopy (HAFM) imaging system. Initial system performance is provided and cantilever dynamics are investigated. HAFM results of human fibroblasts and rat hippocampal neurons under graded levels of hyperbaric gases (up to 6 atmospheres absolute; ATA) are shown. Hyperbaric AFM provides the capability to study the cellular and molecular mechanisms of pressure-dependent disorders.
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Intracellular Localisation of Fluorescently Labelled Nanoparticles
May. 04, 2011

Intracellular Localisation of Fluorescently Labelled Nanoparticles

A laser scanning microscope collects information from a thin focal plane, disregarding out-of-focus information. It has become the standard imaging method to characterise cellular morphology and structures, both in static and living samples. Laser scanning microscopy at high resolution combined with digital image restoration is also a powerful tool to analyse intracellular localisation of fluorescently labelled nanoparticles (NPs), such as their colocalisation within membrane-bound compartments (e.g. more
ESEM in Plant Sciences - Versatile Tool to Study Native & Hydrated Plant Surfaces
Apr. 11, 2011

ESEM in Plant Sciences - Versatile Tool to Study Native & Hydrated Plant Surfaces

ESEM (Environmental Scanning Electron Microscopy) enables the investigation of native, hydrated and uncoated plant surfaces without further sample preparation and the in situ observation of dynamic processes at SEM resolution. A selection of representative plant samples and applications will be presented to show that ESEM is a versatile tool in plant science. more
Live-Cell Super-Resolution with dSTORM
Apr. 04, 2011

Live-Cell Super-Resolution with dSTORM

Live-Cell Super-Resolution with dSTORM: A detailed microscopic characterization of cellular structures is important to understand cellular function. Conventional microscopy in some cases is limited by the achievable spatial resolution of about 200 nm in the imaging plane, which is not sufficient to reveal details at the near-molecular level. This is important if the organization of proteins in small organelles, clusters or machineries are studied. more
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