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Microscopic Imaging of Rosette Nanotubes
Dec. 13, 2010

Microscopic Imaging of Rosette Nanotubes

The characterization of self-assembled rosette nanotubes (RNTs), which are soft organic nanomaterials, can be challenging because of their small dimensions (diameter 3-8 nm) and their sensitivity to damage by microscopy techniques. Consequently, proper sample preparation and optimized microscopy conditions are necessary for successful characterization. In this paper, the critical factors that strongly affect the quality of the structural information obtained for RNTs are presented (fig. 1) more
To Bead or Not To Bead?
Nov. 26, 2010

To Bead or Not To Bead?

In a large core microscopy facility, instrument performance can decline over time, introducing optical artifacts or distortions into the data. We have selected two commercially available tools which can be used in any facility to monitor changes in optical performance. Using these tools, we have identified objective and alignment problems not visible through typical image collection. A similar approach could be of great benefit to other core microscopy facilities in quality assurance. more
Photocatalytic Systems Films Structure
Nov. 22, 2010

Photocatalytic Systems Films Structure

Photocatalytic systems based on chitosan with different molecular mass with the addition of pluronic and porfirin are very effective in the tryptophan photooxidation. Photocatalytic activity of such systems depended on the molecular mass of chitosan. This effect can be bound up with the unique surface structure features of chitosan-based systems. In this work atomic force microscopy is involved for such systems analisys.

Chitosan‑based Photocatalytic Systems
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Cellular and Nuclear Mechanics
Nov. 13, 2010

Cellular and Nuclear Mechanics

Atomic force microscopy can be used to characterize cells and cell nuclei mechanically under physiological conditions by local indentation. Mechanical properties of cells and cell nuclei reflect the status of the the actin network and the nuclear lamina respectively. For cells, the elastic modulus can be obtained by fitting the non-linear Hertz model to the force indentation curves. In the case of cell nuclei, an effective spring constant can be obtained from a linear fit to the force curves. more
The Neuromuscular Junction in the Amphioxus Myotomes
Nov. 05, 2010

The Neuromuscular Junction in the Amphioxus Myotomes

The peculiar morphology of amphioxus segmental muscles was studied with great interest for more than a century. This was mainly due to the possible relation of the amphioxus to the ancestral chordates and vertebrates [6]. These muscles constitute the bulk of the animal consisting of about sixty V-shaped segments (myomeres or myotomes) separated by thin connective tissue septa called myosepta or myocommata [6, 15]. more
Correction of Chromatic Aberration
Nov. 01, 2010

Correction of Chromatic Aberration

Chromatic aberration is one of the main image defects in optical systems. This is caused by the dispersion (i.e. the change in refractive index with wavelength) of any optical media. As a result, a simple lens can be perfectly focused for one wavelength, but badly out of focus for other wavelengths. This defect must be controlled, particularly in demanding fields such as microscopy. Achromatic and apochromatic systems offer suitable solutions. more
Dynamic Positioning with Nanometer Accuracy
Oct. 25, 2010

Dynamic Positioning with Nanometer Accuracy

The best possible positioning accuracy is essential in many technical applications. Examples range from semiconductor manufacturing and biotechnology to optical metrology, microscopy and other imaging methods. Nanopositioning systems with piezoelectric drives are qualified for such applications and due to their short response times and good repeatability mean there is often no serious alternative.
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Light Sources for Epifluorescence Microscopy
Oct. 18, 2010

Light Sources for Epifluorescence Microscopy

If one could „bake" an ideal light source for widefield epifluorescence microscopy, the recipe would include many ingredients of the LED-based solutions which have recently hit the market: they are compact, long-lived and stable, they can be turned on and off in microseconds, their intensity is easily controlled and they are reasonably bright when large areas are to be illuminated under a microscope. Nevertheless, there are still many areas where arc lamps are a better choice. more
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