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Abnormal Cells Under Investigation
Jul. 05, 2010

Abnormal Cells Under Investigation

Fluorescence microscopy combines the power of high performance optical components and digital image analysis. The emission signals of fluorescent dyes show a significant spectral crosstalk which inevitably increases with the number of fluorescent dyes used. However, it is possible to significantly reduce the effects of spectral crosstalk by the application of spectral imaging and spectral unmixing techniques. Spectral imaging is the combination of computer vision and spectroscopy. Epidemiology of Breast Cancer more
AFM and Combined Optical Techniques
Jun. 25, 2010

AFM and Combined Optical Techniques

This article briefly describes the basics of both optical and atomic force microscopy, followed by a discussion of some of the technical challenges of integrating these two distinct imaging modalities. In certain cases, the benefits and disadvantages of different approaches to design and integration are discussed. Lastly, a few examples of successful application of these combined imaging modalities are presented. more
Single Carbon Atom Chains
Jun. 23, 2010

Single Carbon Atom Chains

80kV- High Resolution Transmission Electron Microscopy (HRTEM) characterization of micromechanically cleaved graphene shows in real- time atomically resolved dynamics of the carbon atoms in graphene constrictions. During electron irradiation, processes of hole formation generate graphene constrictions and eventually lead to stable single carbon atom chains. Carbon chains also occur between carbonaceous adsorbates and looping along graphene edges. more
dSTORM
May. 03, 2010

dSTORM

Optical microscopes are subject to the diffraction barrier of light which imposes an optical resolution limit of approximately 200 nm in the imaging plane. dSTORM enables super-resolution imaging with commercially available standard fluorophores and probes at a resolution of ~ 20 nm even in living cells. more
Formation of α-Manganese Dioxide Nanotubes
Apr. 19, 2010

Formation of α-Manganese Dioxide Nanotubes

Hydrothermal decomposition of KMnO4 in the acidic environment in the presence of Fe3+(aq) ions leads to the formation of α-MnO2 nanotubes. The morphology evolution from the sponge-like spheres to the nanotubes as a function of reaction time was imaged by FE-SEM and TEM. The presence of iron in nanotubes was unambiguously confirmed by STEM-EELS measurement across the nanotube diameter.

Introduction
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EDS Using an SDD with X-ray Optic
Mar. 31, 2010

EDS Using an SDD with X-ray Optic

Organic and mineral samples, containing light elements like oxygen, nitrogen or beryllium have been analyzed using an attachable X-ray optic with an SDD in SEM. Compared to the measurements without the additional optic, there is a significant increase of the detection sensitivity for X-rays in the energy range below 1 keV. In contrast the spectrum above 1 keV is not affected. The optic can provide similar intensities for low and high energy X-ray lines even at high accelerating voltage. Introduction more
Breaking the X-ray Resolution Limit
Mar. 24, 2010

Breaking the X-ray Resolution Limit

We have shown that 50 nm details can be distinguished in x-ray images using a compact laboratory set-up. A novel emitter design inside the x-ray source produces a very small, stable focal spot and prevents diffraction limiting effects. Due to unlimited depth of focus, the source doesn't impose a limit on the specimen size that can be reconstructed by 3D x-ray nano-tomography. Introduction more
Perspectives of Superresolution in Neurobiology
Feb. 22, 2010

Perspectives of Superresolution in Neurobiology

Fully understanding the functionality and the complexity of the human central nervous system remains as one of the major open questions in modern science. The Drosophila neuromuscular junction (NMJ) is a widely used and acknowledged model for the analysis of synapse structure and assembly. When considering the size of a Drosophila NMJ synapse of about 500 nm in diameter it appears logical that in order to visualize its spatial architecture the resolution of image acquisition methods needs to be accordingly high. more
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