Sep. 27, 2010
A comprehensive understanding of cellular function on the microscopic level in a whole cell or organism is the ultimate goal in system biology. Where system biology methods isolate individual molecular clusters for further investigations , microscopy offers the potential to directly "see" the complexes  ideally in their natural environment . moreSep. 23, 2010
Live-Cell Assays are used to study the dynamic functional cellular processes in High-Content Screening of drug discovery processes. The large amount of image data created during the screening requires automatic image-analysis procedures that can describe these dynamic processes. One class of tasks in this application is the tracking of cells and the description of the events and the changes in the cell characteristics, so that the desired information can be extracted from it based on data-mining and knowledge-discovery methods. moreSep. 20, 2010
Investigations on nanopositioning and nanomeasuring machines (NPM) are aimed at measurement and manipulation of materials on a nanometer-scale. This requires an exact positioning and calibration of the used tools or Atomic Force Microscopy (AFM) tips and the possibility to retrieve a small area of interest (nm) on a large sample (µm - mm). A possibility to achieve a calibration on the atomic scale can be the use of well known lattice plane distances with high accuracy.
Nanopositioning and Nanomeasuring Machines moreSep. 13, 2010
The formation of CuInSe2 and Cu2ZnSnSe4 thin films in the process of selenization precursor films was studied in use of combined microscopic (HRSEM) and spectroscopic (micro-XRF, EDS, micro-Raman and XRD) methods. moreSep. 06, 2010
In this article we present a universal method for structural investigation of chitin by using correlative AFM-TEM microscopy. The advantage of this method is that polysaccharide chains, proteins, and biominerals can be analyzed simultaneously by AFM (protein-chitin fibril distribution) and analytical TEM (chemical composition of the same particular area).
Introduction moreAug. 30, 2010
In (HR)TEM images of catalysts, it is often difficult to recognize metal nanoparticles with a diameter around 1 nm or below on strongly scattering crystalline oxides. For such systems, the combination of information obtained with an aberration-corrected STEM microscope equipped with BF, DF and secondary electron detectors provides a superior means to detect the nanoparticles, as demonstrated here for Pt particles on cerium oxide. moreAug. 16, 2010
Holographic optical tweezers (HOT) are a versatile tool allowing for the generation of complex arrays of multiple optical traps which act as force sensors with piconewton resolution. The combination of HOT and stop-flow microfluidics integrated with a fluorescence microscope gives full spatial, chemical and visual control over the microenvironment. This allows us to investigate the dynamic properties of complex biosystems at the microscale, such as crosslinking in biomimetic actin networks.
Creating Biomimetic in vitro Models moreAug. 10, 2010
The analysis of the HER2 status is a prerequisite for antibody (and kinase-inhibitor) based, target specific therapy in breast cancer. The diagnosis is usually done on protein level by immunohistochemistry (IH) and/or on DNA level by (Fluorescence)-in-situ-Hybridization (ISH, FISH). Strong HER2 overexpression scored 3+ (on a scale ranging from 0 over 1+, 2+ to 3+) by IH is most often based on HER2 gene amplification and either diagnostic finding is basically indicative for Trastuzumab therapy of breast cancer patients. more