Sep. 06, 2010
In this article we present a universal method for structural investigation of chitin by using correlative AFM-TEM microscopy. The advantage of this method is that polysaccharide chains, proteins, and biominerals can be analyzed simultaneously by AFM (protein-chitin fibril distribution) and analytical TEM (chemical composition of the same particular area).
Introduction moreAug. 30, 2010
In (HR)TEM images of catalysts, it is often difficult to recognize metal nanoparticles with a diameter around 1 nm or below on strongly scattering crystalline oxides. For such systems, the combination of information obtained with an aberration-corrected STEM microscope equipped with BF, DF and secondary electron detectors provides a superior means to detect the nanoparticles, as demonstrated here for Pt particles on cerium oxide. moreAug. 16, 2010
Holographic optical tweezers (HOT) are a versatile tool allowing for the generation of complex arrays of multiple optical traps which act as force sensors with piconewton resolution. The combination of HOT and stop-flow microfluidics integrated with a fluorescence microscope gives full spatial, chemical and visual control over the microenvironment. This allows us to investigate the dynamic properties of complex biosystems at the microscale, such as crosslinking in biomimetic actin networks.
Creating Biomimetic in vitro Models moreAug. 10, 2010
The analysis of the HER2 status is a prerequisite for antibody (and kinase-inhibitor) based, target specific therapy in breast cancer. The diagnosis is usually done on protein level by immunohistochemistry (IH) and/or on DNA level by (Fluorescence)-in-situ-Hybridization (ISH, FISH). Strong HER2 overexpression scored 3+ (on a scale ranging from 0 over 1+, 2+ to 3+) by IH is most often based on HER2 gene amplification and either diagnostic finding is basically indicative for Trastuzumab therapy of breast cancer patients. moreAug. 02, 2010
Collagen is the most abundant protein in the human or animal body. It is found in ligaments, tendons, skin, cartilage, bones, cornea, sclera (white of the eye), and blood vessels to name but a few. All of these tissues are biomechanically important; therefore, knowing the mechanical properties of collagen at all hierarchical levels or length-scales is of critical importance. Our studies have concentrated on the mechanics of collagen fibrils from bovine Achilles tendon, which is a self-assembly of aligned monomeric tropocollagen molecules. moreJul. 26, 2010
Part 1: Deconvolution is an image restoration technique which improves image contrast, resolution and signal to noise ratio. In modern optical microscopy and biological research deconvolution is becoming a fundamental processing step which allows for better image analysis. Deconvolution remains however a challenging task as the result depends strongly on the algorithm chosen, the parameters settings and the kinds of structures in the processed dataset. moreJul. 19, 2010
We report on the development and testing of an integrated web-based project- and access-management system (PAMS) to improve the management of shared resources by technical platforms in a scientific research environment. moreJul. 05, 2010
Fluorescence microscopy combines the power of high performance optical components and digital image analysis. The emission signals of fluorescent dyes show a significant spectral crosstalk which inevitably increases with the number of fluorescent dyes used. However, it is possible to significantly reduce the effects of spectral crosstalk by the application of spectral imaging and spectral unmixing techniques. Spectral imaging is the combination of computer vision and spectroscopy.
Epidemiology of Breast Cancer more