Nov. 03, 2009
Ever since the first human embryonic stem cell line was established, hopes have been raised that treatment for a wide range of cellular degenerative disease and injuries is imminent. While significant progress has been made in stem cell research in recent years, several hurdles remain to be overcome before stem cells can be used routinely in the clinic. In particular, the understanding of the mechanisms that control cell integration, differentiation and proliferation in recipient tissues is far from complete. moreNov. 03, 2009
In this article we discuss the ability of optical tweezers to both trap and probe airbrone particles. We highlight that a number of optical trapping techniques such as holographic optical tweezers and dual beam traps are possible and may open up new ways to analyse aerosols, for atmospheric studies. We show that both liquid and solid aerosols can be trapped and that even broadband laser sources such as a supercontinuum may be used to trap particles and can probe properties such as evaporation. moreNov. 03, 2009
An efficient receptor-mediated delivery system has been developed using preformed complexes of Quantum Dots (QDs) and the Epidermal Growth Factor (EGF) ligand. Liposomes are either loaded or surface modified combining two colors of QDs probes. The dual labeling strategies involve only biotin-streptavidin interactions and do not require further purification from free QDs-EGF. moreNov. 03, 2009
Fluorescence microscopy has become the method of choice in the majority of life-science applications. However, standard fluorophores do not luminesce brightly enough for some applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with a wide field of observation and a large depth of focus. moreNov. 03, 2009
Nowadays, deconvolution in cell and tissue imaging has matured into a standard restoration technique that is accessible to large fraction of the microscopy community thanks to steadily improving algorithms. Still, deconvolution is often the rate-limiting step in the analysis of the acquired data, even at today's computer performance. Here, we present the Huygens Remote Manager, an open-source, efficient, multi-user web-based interface for parallel batch deconvolutions. moreNov. 03, 2009
Superresolution methods in digital holographic microscopy provide a useful tool to overcome the Abbe‘s diffraction limit when using modest microscope lenses. The process improves the cutoff frequency of the microscope lens by means of the generation of a synthetic aperture based on time multiplexing and using 3 main stages: optical coding, optical decoding, and digital post-processing. After the whole process, a superresolved image is obtained by Fourier transformation of the synthetic aperture.
The Limited Resolving Power of Imaging Systems moreNov. 03, 2009
The Langmuir-Blodgett (LB) thin film deposition technique [1-2] is a suitable method to form monolayer or multilayer organic thin films on the nanoscale. Other advantages of LB films are follows as: the film thickness and molecular architecture can easily be controlled, a centrosymmetric or non-centrosymmetric ultra-thin films can be fabricated using LB film fabrication procedures. Therefore these organic thin films have many potential applications in physics, chemistry, biology and molecular electronics. moreNov. 03, 2009
Confocal technique and white light interferometry have demonstrated to be suitable for characterization of transparent thick films. Layer's thickness and 3D topographies of its upper and lower interfaces can be determined from the two peaks in the confocal axial response or from the two sets of interference fringes developed during a vertical scan. Refraction index mismatch between immersion medium and layer worsens the performance of these techniques when profiling lower surface.
Surface Profiling Techniques more