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Nanomechanics of Collagen Fibrils
Aug. 02, 2010

Nanomechanics of Collagen Fibrils

Collagen is the most abundant protein in the human or animal body. It is found in ligaments, tendons, skin, cartilage, bones, cornea, sclera (white of the eye), and blood vessels to name but a few. All of these tissues are biomechanically important; therefore, knowing the mechanical properties of collagen at all hierarchical levels or length-scales is of critical importance. Our studies have concentrated on the mechanics of collagen fibrils from bovine Achilles tendon, which is a self-assembly of aligned monomeric tropocollagen molecules. more
Comparison of Deconvolution Software in 3D Microscopy
Jul. 26, 2010

Comparison of Deconvolution Software in 3D Microscopy

Part 1: Deconvolution is an image restoration technique which improves image contrast, resolution and signal to noise ratio. In modern optical microscopy and biological research deconvolution is becoming a fundamental processing step which allows for better image analysis. Deconvolution remains however a challenging task as the result depends strongly on the algorithm chosen, the parameters settings and the kinds of structures in the processed dataset. more
Enhancing the Efficiency of Resource Use
Jul. 19, 2010

Enhancing the Efficiency of Resource Use

We report on the development and testing of an integrated web-based project- and access-management system (PAMS) to improve the management of shared resources by technical platforms in a scientific research environment. more
Abnormal Cells Under Investigation
Jul. 05, 2010

Abnormal Cells Under Investigation

Fluorescence microscopy combines the power of high performance optical components and digital image analysis. The emission signals of fluorescent dyes show a significant spectral crosstalk which inevitably increases with the number of fluorescent dyes used. However, it is possible to significantly reduce the effects of spectral crosstalk by the application of spectral imaging and spectral unmixing techniques. Spectral imaging is the combination of computer vision and spectroscopy. Epidemiology of Breast Cancer more
AFM and Combined Optical Techniques
Jun. 25, 2010

AFM and Combined Optical Techniques

This article briefly describes the basics of both optical and atomic force microscopy, followed by a discussion of some of the technical challenges of integrating these two distinct imaging modalities. In certain cases, the benefits and disadvantages of different approaches to design and integration are discussed. Lastly, a few examples of successful application of these combined imaging modalities are presented. more
Single Carbon Atom Chains
Jun. 23, 2010

Single Carbon Atom Chains

80kV- High Resolution Transmission Electron Microscopy (HRTEM) characterization of micromechanically cleaved graphene shows in real- time atomically resolved dynamics of the carbon atoms in graphene constrictions. During electron irradiation, processes of hole formation generate graphene constrictions and eventually lead to stable single carbon atom chains. Carbon chains also occur between carbonaceous adsorbates and looping along graphene edges. more
dSTORM
May. 03, 2010

dSTORM

Optical microscopes are subject to the diffraction barrier of light which imposes an optical resolution limit of approximately 200 nm in the imaging plane. dSTORM enables super-resolution imaging with commercially available standard fluorophores and probes at a resolution of ~ 20 nm even in living cells. more
Formation of α-Manganese Dioxide Nanotubes
Apr. 19, 2010

Formation of α-Manganese Dioxide Nanotubes

Hydrothermal decomposition of KMnO4 in the acidic environment in the presence of Fe3+(aq) ions leads to the formation of α-MnO2 nanotubes. The morphology evolution from the sponge-like spheres to the nanotubes as a function of reaction time was imaged by FE-SEM and TEM. The presence of iron in nanotubes was unambiguously confirmed by STEM-EELS measurement across the nanotube diameter.

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