Aug. 02, 2010
Collagen is the most abundant protein in the human or animal body. It is found in ligaments, tendons, skin, cartilage, bones, cornea, sclera (white of the eye), and blood vessels to name but a few. All of these tissues are biomechanically important; therefore, knowing the mechanical properties of collagen at all hierarchical levels or length-scales is of critical importance. Our studies have concentrated on the mechanics of collagen fibrils from bovine Achilles tendon, which is a self-assembly of aligned monomeric tropocollagen molecules. moreJul. 26, 2010
Part 1: Deconvolution is an image restoration technique which improves image contrast, resolution and signal to noise ratio. In modern optical microscopy and biological research deconvolution is becoming a fundamental processing step which allows for better image analysis. Deconvolution remains however a challenging task as the result depends strongly on the algorithm chosen, the parameters settings and the kinds of structures in the processed dataset. moreJul. 19, 2010
We report on the development and testing of an integrated web-based project- and access-management system (PAMS) to improve the management of shared resources by technical platforms in a scientific research environment. moreJul. 05, 2010
Fluorescence microscopy combines the power of high performance optical components and digital image analysis. The emission signals of fluorescent dyes show a significant spectral crosstalk which inevitably increases with the number of fluorescent dyes used. However, it is possible to significantly reduce the effects of spectral crosstalk by the application of spectral imaging and spectral unmixing techniques. Spectral imaging is the combination of computer vision and spectroscopy.
Epidemiology of Breast Cancer moreJun. 25, 2010
This article briefly describes the basics of both optical and atomic force microscopy, followed by a discussion of some of the technical challenges of integrating these two distinct imaging modalities. In certain cases, the benefits and disadvantages of different approaches to design and integration are discussed. Lastly, a few examples of successful application of these combined imaging modalities are presented. moreJun. 23, 2010
80kV- High Resolution Transmission Electron Microscopy (HRTEM) characterization of micromechanically cleaved graphene shows in real- time atomically resolved dynamics of the carbon atoms in graphene constrictions. During electron irradiation, processes of hole formation generate graphene constrictions and eventually lead to stable single carbon atom chains. Carbon chains also occur between carbonaceous adsorbates and looping along graphene edges. moreMay. 03, 2010
Optical microscopes are subject to the diffraction barrier of light which imposes an optical resolution limit of approximately 200 nm in the imaging plane. dSTORM enables super-resolution imaging with commercially available standard fluorophores and probes at a resolution of ~ 20 nm even in living cells. moreApr. 19, 2010
Hydrothermal decomposition of KMnO4 in the acidic environment in the presence of Fe3+(aq) ions leads to the formation of α-MnO2 nanotubes. The morphology evolution from the sponge-like spheres to the nanotubes as a function of reaction time was imaged by FE-SEM and TEM. The presence of iron in nanotubes was unambiguously confirmed by STEM-EELS measurement across the nanotube diameter.