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Wide-field CARS-Microscopy
Nov. 01, 2008

Wide-field CARS-Microscopy

Coherent anti-Stokes Raman scattering (CARS) microscopy is a branch of nonlinear microscopy that allows chemical imaging of targeted vibrational transitions in unstained samples. A resonantly enhanced blue-shifted CARS signal is generated from NIR or visible light, thus the method is more sensitive than normal Raman microscopy and offers better resolution than IR microscopy. CARS microscopes are mostly set up as confocal scanning microscopes, but wide-field approaches are possible as well.

Brief Introduction to CARS-microscopy
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3D Orientation Microscopy
Nov. 01, 2008

3D Orientation Microscopy

Combining electron backscatter diffraction (EBSD), a scanning electron microscope (SEM) and a focused ion beam (FIB) together into a single instrument enables three dimensional (3D) characterization of microstructure in crystalline materials. Combining these techniques together has enormous potential in materials science.

Electron Backscatter Diffraction

Traditionally, microstructure refers to features that are visually evident in an optical or electron microscope. However, many critical aspects of microstructure are not visually evident. more
Ultrasonic Nanofabrication with an AFM
Nov. 01, 2008

Ultrasonic Nanofabrication with an AFM

Ultrasonic AFM may improve fabrication technologies on the nanometer scale. In the presence of ultrasonic vibration, hard surfaces can be indented and scratched with the tip of a soft cantilever, due to its inertia. Ultrasound reduces or even eliminates friction, and hence modifies the tip-nanoparticle-surface interactions in AFM manipulation. The subsurface sensitivity of the technique makes feasible the purposed manipulation of subsurface nanoscale features by ultrasonic actuation.

Ultrasonic Atomic Force Microscopies
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FIB Milling and Canneling
Nov. 01, 2008

FIB Milling and Canneling

Focused Ion Beam instruments (FIB) are used for the preparation of electron microscopy specimens and for the fabrication of nano and micro components. Using polycrystalline Cu as an example, the influence of the crystallographic orientation, as obtained by EBSD, on the milling result is demonstrated. Different milling rates are due to the channeling effect. With some orientations a topography with characteristic features, like ripples, is generated, which were quantified using AFM images. more
Verifying Engineering at the Nanoscale
Nov. 01, 2008

Verifying Engineering at the Nanoscale

Packaging drugs and genes into nanoparticles enables drug or gene biodistribution to be favourably altered, with an ultimate therapeutic benefit [1-3]. To acquire such control on the in vivo fate of drugs and genes requires that such particles be precision engineered and electron microscopy is one of the techniques used to visualise and confirm the results of such engineering.

Methods
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Enabling 360 degree TEM/STEM  of Nanoparticles
Nov. 01, 2008

Enabling 360 degree TEM/STEM of Nanoparticles

A new protocol for functionalizing sample holders has been developed for 360° TEM/STEM observation of nanoparticles and nanostructures. The three step process includes FIB milling to customize sample stub geometry, thin film deposition for substrate selection and subsequent chemical functionalization for nanoparticle adhesion. This protocol was used to determine the morphology and local material properties of individual Au/SiO2 core-shell nanoparticles used in a DNA detection assay.

Nanoscience Imaging & Spectroscopy
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Systematic Analysis of FRAP Experiments
Nov. 01, 2008

Systematic Analysis of FRAP Experiments

Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients

Introduction
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Fundamental Knowledge - Part 4
Sep. 01, 2008

Fundamental Knowledge - Part 4

Fundamental Knowledge - Part 4: Digitial Imaging Cameras: Deciphering Specifications. In the first instalments of this article series we looked at the theory behind microscopy: how a small facet of a sample can be magnified and focused efficiently and effectively at the focal plan of the user's eyes or the imaging device. In this article, we look at the surprisingly complex world of digital microscopy cameras and how to find the best one for each research task.
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