Aug. 01, 2008
Quantitative Phase Microscopy is a simple imaging methodology for resolving internal morphology of unstained living specimens. It computes the 2D phase images of a specimen from a combination of bright field images. However, when used in conjunction with polarised light can lead to the simultaneous mapping of the phase and the retardation of an optically anisotropic specimen. Experimentally the procedure is implemented without the need of expensive compensators used in traditional methods. moreAug. 01, 2008
Liver cirrhosis can be due to various causes, including alcohol abuse and viral hepatitis B. Two of the most frequent hereditary metabolic diseases are also indicated: hemochromatosis with pathological iron accumulation and Wilson's Disease with pathological copper accumulation. In order to distinguish them, the Institute of Pathology of the University of Rostock uses energy-filtering transmission electron microscopes for investigation of tissue sections. moreAug. 01, 2008
DualBeam systems that combine a focused ion beam (FIB) with a scanning electron microscope (SEM) in a single instrument provide valuable rapid prototyping capability that can significantly shorten development times for nanoscale devices in a variety of applications. The DualBeam's strength in prototyping applications derives from its ability to simultaneously create and observe structures with nanoscale resolution. The FIB can remove (mill) and deposit material in complex patterns with dimensional control of a few nanometers. moreAug. 01, 2008
Cryo-electron tomography was used for studying the molecular organization of the cytoplasm in intact cells. Flat areas of cells grown on EM grids and preserved by rapid freezing within milliseconds were imaged in 3D. In the resulting maps the selected morphological and structural features were analyzed at unprecedented detail, and frequently correlated to light-microscopy images. We portray here host cell-virus interactions, the morphology of neurons and malaria parasites.
Analysis of Cellular Structures and Processes moreAug. 01, 2008
CryoEM can be divided into three different sub-disciplines: electron crystallography where the macromolecules are arranged periodically into thin crystals, single particle cryoEM of randomly oriented isolated macromolecular assemblies and electron tomography of vitreous specimens containing objects in situ or in isolated form but being reconstructed individually. These methods provide a comprehensive, complementary approach of transmission electron microscopy (TEM) for studies of biological structure reaching from the atomic to the cellular level. moreAug. 01, 2008
Applications of electron microscopy, stand-alone and in combination with other microscopy methods, in diverse fields of biological and medical research as well as in medical diagnosis will be one of the main topics at the Microscopy Conference (MC 2007), to be held from 2 to 7 September 2007 at the Saarland University in Saarbrücken (Germany). This is the 33rd Conference of the Deutsche Gesellschaft für Elektronenmikroskopie e. V. (DGE), which was initially conceived as a national meeting but has achieved in recent years a remarkable international character. moreAug. 01, 2008
A precise design of complex microstructural features calls for adequate 3D characterization. For the example an automatic classification of local graphite morphologies was developed for application of advanced cast iron in high performing functional gradient materials. FIB/SEM Nanotomography was combined with a computer simulation to measure a reliable probability distribution of random 2D sections through reconstructed 3D particles. The procedure is applicable for other complex microstructures.
moreAug. 01, 2008
Scanning force microscopy has become in the last two decades the most widely applied method within the field of scanning probe microscopy. In certain cases atomic resolution can be obtained and a lot of specialised modes of application allow the local detection of a variety of probe-sample-interactions. This opens the possibility to analyse physical, chemical and even biological phenomena with an unprecedented sensitivity and resolution.
Visualising Magnetic Domains more