Nov. 04, 2009
Normal and diseased tissues are complex mixtures of different cell populations. A better understanding of protein expression changes that occur during
diseases needs sensitive and specific technologies for each of these cell types. Mass spectrometry based tissue imaging (MALDI-MSI) is a newly developed technique, allowing the visualization of proteins, peptides, lipids and small molecules directly on thin sections cut from fresh frozen or fixed paraffin embedded tissues. moreNov. 04, 2009
The high resolution and sensitivity of electron microscopy is a valuable ancillary tool or gold standard in pathological diagnosis. The conventional sample turnaround time for processing in the lab can be significantly reduced from days to hours by the microwave technology. Microwave-assisted tissue processing, in combination with digital image acquisition, enables a "same-day" diagnosis in urgent clinical cases. Ultrastructural telepathology allows instant and live second opinion retrieval from a remote expert worldwide.
Introduction moreNov. 04, 2009
We use the intense light coming from a third generation synchrotron source to perform computed tomography (CT) with micro-resolution and high material contrast. This unique tool allows for shedding light on methodical errors which are involved when applying classical histomorphometry. The tomographic approach using X-rays is not only non-destructive, it also allows for an analysis in a true 3D manner. Thus, it delivers knowledge of which is important to fully understand e. g. bone regeneration.
Introduction moreNov. 03, 2009
For more than 30 years - on a worldwide basis - the Ergonom technology by Kurt Olbrich has been in use successfully among insiders. They see it as an excellently qualified light-microscope for most complicated tasks. At present, these special microscopes are being offered to a larger group of top microscopists. moreNov. 03, 2009
"We are now able to take a series of photographs at slightly different focal planes or levels through a single cell or group of cells. These photographs result in a consecutive record of the internal architecture of the cell or cells. This accomplishment we have called "optical sectioning". ... By means of this new development a transparent specimen such as a group of cells may be sectioned optically. ... Detail above or below the focal plane does not interfere."  moreNov. 03, 2009
In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. moreNov. 03, 2009
The development of instrumentation for microscopy has advanced enormously in recent years and one of the critical advancements has been the development of low voltage scanning electron microscopy (LVSEM) that allows high-resolution imaging of delicate biological structures with minimal coating requirements. LVSEM provides resolution at levels that previously could only be achieved with transmission electron microscopy (TEM). moreNov. 03, 2009
The appearance of hair is influenced by the 3D geometric arrangement of hair fibres. An accurate 3D representation of hair is needed for hair research to assess the effect of different treatments. X-ray microtomography (XRT) can be used for the 3D visualisation of hair arrays and the quantitative analysis of volume and shape attributes.