Mar. 01, 2008

DNA Chip at a Molecular Scale

Atomic Force Microscopy becomes an invaluable technique for the study of biological systems at a nanometric scale [1, 2]. During the past decade, it has been increasingly used to image and manipulate biomolecules and cell surfaces in air or in situ. Development of dynamic modes of operation adapted for the imaging of soft samples has significantly contributed to overcome the usual issue of damage done to these biological objects by the scanning tip [3].

Applications To Biology
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Tip-enhanced Raman Spectroscopy and Imaging

A possibility to not only visualize but to locally probe a chemical structure, composition, conformational state and stresses on the nanoscale has stimulated the development of apertureless near-field vibrational spectroscopy and imaging with ultrahigh spatial resolution laying beyond the diffraction limit [1-3]. It has become possible due to the delocalization of evanescent waves (near-field) existing in the proximity of nano-sized objects with a sharp metal probe.
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High-pressure Freezing in CLEM

Research methods using so-called Correlative Light Electron Microscopy (CLEM) techniques have gained increasing attention in recent years. Most techniques have focused on the light microscopy part of the process. The processing for electron microscopy, however, deserves attention as well to get the most out of the technique. Some new developments, including the use of a superior fixation technique, are described. moreMar. 01, 2008

Flame Sprayed Zinc Coatings

Zinc thermal spraying is widely used for steel coatings. In the present work, the morphology and the corrosion performance of the as-formed coatings is investigated. Initial observations took place with a low magnification stereoscope equipped with a CCD camera, while Scanning Electron Microscopy (SEM) was used for more detailed observation. ­Selected samples were also examined with High Resolution Electron Microscopy (HREM) in order to investigate the oxygene distribution which was detected by EDS analysis. moreJan. 01, 2008

Advanced Live Cell Imaging

Special Edition of Imaging & Microscopy For Single Articles: Please Click on the Headline to Download the PDF
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Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data

Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data. Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients. moreNov. 01, 2007

Air Quality Control for Hazardous Bio-Material Automatic Probe Handling, Image Acquisition & Image ...

Air Quality Control for Hazardous Bio-Material Automatic Probe Handling, Image Acquisition & Image Analysis. Airborne microorganisms are ubiquitously present in various indoor and outdoor environments. The potential implication of fungal contaminants in bio-aerosols on occupational health is recognized as a problem in several working environments. There is a concern on the exposure of workers to bio-aerosols especially in composting facilities, in agriculture, and in municipal waste treatment. moreNov. 01, 2007

Wide-field CARS-Microscopy: Functional Imaging at a Glimpse

Wide-field CARS-Microscopy: Functional Imaging at a Glimpse. Coherent anti-Stokes Raman scattering (CARS) microscopy is a branch of nonlinear microscopy that allows chemical imaging of targeted vibrational transitions in unstained samples. A resonantly enhanced blueshifted CARS signal is generated from NIR or visible light, thus the method is more sensitive than normal Raman microscopy and offers better resolution than IR microscopy. CARS microscopes are mostly set up as confocal scanning microscopes, but wide-field approaches are possible as well. more