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Deep 3D Optical Metrology
Nov. 03, 2009

Deep 3D Optical Metrology

Confocal technique and white light interferometry have demonstrated to be suitable for characterization of transparent thick films. Layer's thickness and 3D topographies of its upper and lower interfaces can be determined from the two peaks in the confocal axial response or from the two sets of interference fringes developed during a vertical scan. Refraction index mismatch between immersion medium and layer worsens the performance of these techniques when profiling lower surface.

Surface Profiling Techniques
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Current-sensing AFM Study
Nov. 03, 2009

Current-sensing AFM Study

The easiest imaging mode for measuring the local conductivity of a sample is to combine the current measurements with contact mode Atomic Force Microscopy (AFM) imaging. Current-Sensing Atomic Force Microscopy (CS-AFM) is a powerful technique for electrical characterization of conductivity variation in resistive samples. It allows direct and simultaneous visualization of the topography and current distribution of a sample. In the studies presented in this report the CS-AFM technique has been implemented to study Cr contact formation on the 6H-SiC(0001). more
Automatic Quality Control of Cereals
Nov. 03, 2009

Automatic Quality Control of Cereals

The increasing demands on the quality and consumption safety of grain imply, for food economy, increased expenditures for product and process supervision. Within the framework of the control of goods received and quality of the processing industries, various laboratory methods are applied at present to check the properties of the products and the observance of the standards. more
Correlative AFM and TEM of Soft Material
Nov. 03, 2009

Correlative AFM and TEM of Soft Material

We describe a comprehensive characterization of biological and polymer samples, when a particular organelle or macromolecule cluster is cut into two parts: One part is used for AFM and the other for TEM. Information about the microstructure of the sample (from TEM) in combination with the data on distribution, morphology and mechanical properties of macromolecular/chain content (from AFM) reveals new structural aspects going beyond the possibilities offered by AFM or TEM alone.

What is more valid: information obtained by eyes or by hand?
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C‑AFM and X‑TEM
Nov. 03, 2009

C‑AFM and X‑TEM

Thin intrinsic silicon films containing microcrystalline grains embedded in amorphous tissue were studied by two complementary microscopy techniques. The conductive atomic force microscopy was performed in standard ambient conditions with very sensitive (pA) current detection. The cross-sectional transmission electron microscopy images of the amorphous phase revealed the columnar structure, which was attributed to the bumpy structures on the surface.

Introduction
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M-FISH and QuantiFISH
Nov. 02, 2009

M-FISH and QuantiFISH

Malignant cell growth is acquired by genetic and / or chromosomal instability e. g. gene amplification or deletion, chromosomal polysomy, aneuploidy or translocations. Hence, molecular and cytogenetic analysis has become an essential as well as indispensable complementing tool in routine diagnostics of histological sections. more
Selective Harmonic Optical Microscopy
Nov. 02, 2009

Selective Harmonic Optical Microscopy

SHG is an important contrast mechanism in optical examination for thick biological tissues. Fibrous proteins, such as myosin and collagen, exhibit biophotonic crystal nature and are dominant SHG harmonophores in vertebrates. Local molecule arrangements strongly affect SHG polarization behavior. Here we demonstrate to distinguish myosin-based muscle fibers from intertwined collagenous perimysium through SHG polarization selection, without complicated staining or sample/image processing required. more
Environmental Considerations for Long Term Timelapse Imaging
Dec. 01, 2008

Environmental Considerations for Long Term Timelapse Imaging

Environmental Considerations for Long Term Timelapse Imaging: Many of biology's most interesting questions on the growth, division and apoptosis of living cells can be addressed by microscopic observation using long term timelapse imaging. Live cell imaging experiments pose many challenges, however, not the least of which is keeping cells alive and healthy throughout an experiment. Not only are cells vulnerable to photodamage, but maintaining environmental conditions necessary to keep cells alive on the microscope stage for hours, days or even weeks is far from trivial. more
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