Jun. 01, 2008
In the past decades, high-throughput technologies were developed with the aim to unravel the secret of life. In the genomics project many genomes were sequenced, including the human genome. We ended up with a lot of data but no real clue if and at what point of time a certain illness will manifest, finally not the gene but the presence or absence of the responsible protein will be decisive. Therefore, proteomics came into reach and focus of research. Again we ended up with a wealth of data but still the final answer cannot be found. moreJun. 01, 2008
Microbial biofilm was imaged by SEM in high vacuum mode after standard preparation, and in environmental mode without any sample pre-treatment. ESEM represents an effective technique for detecting high-magnification hydrated biofilm without the introduction of dehydration artifacts, while HVSEM allows easier cell discrimination. The combination of both HVSEM and ESEM information can provide a complete description of the cellular and extracellular matrix components of the biofilm.
Introduction moreJun. 01, 2008
A chain reaction is building in the world of nanotechnology and it is rapidly approaching critical mass. New tools and capabilities are enabling new discoveries, which are creating new products, which are generating new demand, which is funding new tool development... We are on the threshold of an explosion in both our fundamental knowledge and its practical applications.
TEM Improved In Usability moreJun. 01, 2008
Confocal and Atomic Force Microscopy: A wide range of different forms of microscopy may be applied to the visualisation of biological specimens. Confocal microscopy can provide 3D information about fluorescently labelled cells with the added advantage that it excludes out of focus light. Atomic force microscopy (AFM) provides direct high resolution images of surface features of a sample. moreJun. 01, 2008
Transduction of sound is dependent upon the organisation of the cytoskeletal structures that form and support the stereociliary bundle of cochlear hair cells. Freeze-fracture followed by deep etching reveals the organisation of the hair cell cytoskeleton and indicates how mechanical support for stereocilia is provided. This can offer explanations of why mutations in certain genes that are widely expressed in the body have effects only upon the functioning of auditory hair cells to cause non-syndromic deafness.
Introduction moreJun. 01, 2008
Fundamental Knowledge - Part 3: In part one (Fundamental Knowledge - Part 1 ) and two (Fundamental Knowledge - Part 2) of this chapter we looked at the physical principals of light and its interaction with optical surfaces. moreJun. 01, 2008
The unique combined ability of transmission and scanning electron microscopes to visualise the fine structure and interaction of cells, bacteria, viruses and macromolecules has made these instruments essential tools in the fight against potentially life-threatening microbes. moreJun. 01, 2008
Lipid Rafts Mechanical Properties Revealed by AFM: We present a method based on atomic force microscopy which permits to detect and measure the mechanical properties of lipid rafts in living neurons. This technique, based on the interpretation of force distance curves, confirmed that rafts have a diameter lower than 70 nm and that they are stiffer than their surrounding membrane.
Lipid Rafts Play Primordial Role more