Nov. 03, 2009
We describe a comprehensive characterization of biological and polymer samples, when a particular organelle or macromolecule cluster is cut into two parts: One part is used for AFM and the other for TEM. Information about the microstructure of the sample (from TEM) in combination with the data on distribution, morphology and mechanical properties of macromolecular/chain content (from AFM) reveals new structural aspects going beyond the possibilities offered by AFM or TEM alone.
What is more valid: information obtained by eyes or by hand? moreNov. 03, 2009
Thin intrinsic silicon films containing microcrystalline grains embedded in amorphous tissue were studied by two complementary microscopy techniques. The conductive atomic force microscopy was performed in standard ambient conditions with very sensitive (pA) current detection. The cross-sectional transmission electron microscopy images of the amorphous phase revealed the columnar structure, which was attributed to the bumpy structures on the surface.
Introduction moreNov. 02, 2009
Malignant cell growth is acquired by genetic and / or chromosomal instability e. g. gene amplification or deletion, chromosomal polysomy, aneuploidy or translocations. Hence, molecular and cytogenetic analysis has become an essential as well as indispensable complementing tool in routine diagnostics of histological sections. moreNov. 02, 2009
SHG is an important contrast mechanism in optical examination for thick biological tissues. Fibrous proteins, such as myosin and collagen, exhibit biophotonic crystal nature and are dominant SHG harmonophores in vertebrates. Local molecule arrangements strongly affect SHG polarization behavior. Here we demonstrate to distinguish myosin-based muscle fibers from intertwined collagenous perimysium through SHG polarization selection, without complicated staining or sample/image processing required. moreDec. 01, 2008
Environmental Considerations for Long Term Timelapse Imaging: Many of biology's most interesting questions on the growth, division and apoptosis of living cells can be addressed by microscopic observation using long term timelapse imaging. Live cell imaging experiments pose many challenges, however, not the least of which is keeping cells alive and healthy throughout an experiment. Not only are cells vulnerable to photodamage, but maintaining environmental conditions necessary to keep cells alive on the microscope stage for hours, days or even weeks is far from trivial. moreDec. 01, 2008
Nanomaterials for Sustainable Energy: The atomic structure of nanomaterials and the energy needed for their function can be optimised by a fundamental understanding of catalytic behaviour of nanoparticles and of the physical, atomic-level properties of materials for solar cells, fuel cells and light sources. This requires advanced tools that can see down to the individual atoms and sense their chemical environment, show information in three dimensions and allow experiments in situ to follow specific reactions. moreDec. 01, 2008
Zeolites: Highly Resolving Electron Microscopy & Digital Image Processing - Hardly anyone knows what they're called. And we use them every day, even though we may not know it. We're talking about zeolites, a group of minerals with extraordinary and outstanding properties. Since being discovered in 1756, zeolites have been a focus of research. There was a real run on them starting in 1920 once it became possible to decode the crystalline structure of natural zeolites. moreNov. 01, 2008
During the last decade microscopy has gone through a series of major improvements. The demand of microscopy technique has increased a lot and has brought the limits in optical resolution of light microscopes, as described by Ernst Abbe, to be extended by new technologies like 4Pi, STED , deconvolution and others. Scientists want to resolve small compartments and structures within a cell and but at the same time need to visualize a large field of view to be able to understand the complexity of biological organisms. more