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Fluorescence Stereomicroscopy

Mar. 01, 2005
  

Fluorescence Stereomicroscopy. It goes without saying that a new generation of high-performance stereomicroscopes should have the best possible optics. Easier said than done, given that stereomicroscopes based on the telescope principle have been around for almost 60 years and the optics are already pretty sophisticated. The demands that developers were asked to meet therefore sounded almost fanciful: up to 20 % more resolution, even greater contrast and enhanced depth perception in the stereoscopic image. Not only that, the new generation also has to deliver the brightest fluorescence images that have ever been seen with a stereomicroscope. The result is therefore all the more impressive: with the Stereo Lumar.V12 Carl Zeiss is offering the most powerful fluorescence stereomicroscope ever.

Much more is Visible

The Stereo Lumar.V12 has been designed to provide exceptional performance for all of the wide-ranging applications in the field of fluorescence stereomicroscopy. New optics provide the basis for this accomplishment – optics distinguished by the fact that all the important components of the stereomicroscope, such as objective, microscope body with 12.5x zoom and tube, have been rigorously modified to meet the demands of fluorescence microscopy. At comparable reproduction scales, this new stereomicroscope makes it possible to achieve fluorescence imaging with light microscopy quality for the first time. This can be clearly seen using the example of the desmid algae Micrasterias, acquired in UV excitation under entirely comparable technical conditions.

Shining Examples

As the heart of the microscope, the objective has a decisive impact on the optical performance of the overall system. For the Stereo Lumar.V12, Carl Zeiss has drawn on its extensive knowledge and years of experience as market leader in sophisticated fluorescence microscopes to develop new special objectives. Two quick-change objectives, representing a new standard in fluorescence stereomicroscopy, are available: for example, the NeoLumar S 1.5x, with a resolution of 0.6 μm, is particularly well-suited to the observation and documentation of extremely fine fluorescent structures, whilst the NeoLumar S 0.8x, with a free working distance of 80 mm, offers plenty of room for preparation.

Revolutionary Operation

SyCoP (System Control Panel) is a new, compact control unit that has been designed specifically for stereomicroscopy.

It combines different control elements, such as a joystick, buttons and a touch screen, in the convenient design of a computer mouse, enabling easy handling of increasingly complex operations in microscopy. You can now control all the essential functions of the stereomicroscope with speed, precision and reproducibility at the touch of a button, and without having to lift your eyes from the eyepiece. This leaves you free to devote all your attention to the specimen, not to operating the microscope. It will even be possible to integrate future motorised and coded components simply via the open CANBus 29 system and operate them using SyCoP.
And another innovation in the field of microscopy: SyCoP informs the user at a glance about all the important optical parameters of the current microscope setting, such as total magnification, visible object field, maximum possible resolution and depth of focus.

Precision and Perfection

As optical performance increases, so do the demands on the mechanics too. The stand and focusing equipment play a major role here. Stability, sturdiness and sufficient room in the specimen space are as important as rapid, appropriately sensitive and reproducible focusing of the microscope. The newly developed precision mechanics of the motor focusing are as accurate as clockwork. The focus is set rapidly and precisely using SyCoP, if desired, in steps of 350 nm and across a 340 mm travel range. New, highly precise and wear-resistant special plastics help to make this possible. With its unusual stand construction with decentralised profile S column, the Stereo Lumar.V12, featuring a large scratch-resistant stage plate (250 x 410 mm), offers ample room in the specimen space. Fiber-optical and LED components for illumination and contrast can be fitted easily via standardised interfaces, as can gliding, rotating, balland- socket, mechanical and scanning stages.

Summary

The new Stereo Lumar.V12 fluorescence stereomicroscope demonstrably represents the best in optics, innovative operation and mechanical precision. It marks a new peak in the area of high-performance, main-objective stereomicroscopy. And state-of-the-art development and production technologies ensure that all this can be offered at an attractive price.

Keywords: fluorescence stereomicroscopy high-performance stereomicroscopes main-objective stereomicroscopy Stereo Lumar.V12 SyCoP System Control Panel

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