Demonstration of Carbon Nano-Onions Functionalization with Biomolecules by AFM and FT-IR Studies

Small (5-6 nm) oxidized Carbon Nano-Onions (ox-CNOs) can be functionalized with biomolecules due to their good dispersion and relative high reactivity. MTS test on skin fibroblasts proved that CNOs are non-cytotoxic [1]. Therefore, biosensors based on CNOs were fabricated. Covalent binding between sensor layers were possible after functionalization CNOs with carboxylic groups (ox-CNOs) by chemical oxidation using method applied by Lieber et al. [2]. Oxidized CNOs become soluble in an aqueous solution. Functionalization of ox-CNOs with biomolecules by amidation process was based on biotin-avidin interaction [1]. Sensor for avidin was prepared on gold surface by layer-by layer assembly method. Each modification step was monitored by atomic force microscopy (AFM), Fourier Transform Infrared Spectroscopy with Total Attenuated Reflactance Mode (FT-IR/HATR) and Surface Plasmon Resonance (SPR). AFM measurements showed surface shape of each layer, SPR proved changes in refractive index after each step of modification, and FT-IR/HATR confirmed covalent linkage between interacting biomolecules. Results showed, that small-CNOs can be potentially usefull in biosensors developing.

Literature:
[1] Luszczyn J., Plonska-Brzezinska M.E., Palkar A., Dubis A.T., Simionescu A., Simionescu D.T., Kalska-Szostko B., Winkler K., Echegoyen L., Chem. Eur. J. 16, 2010, 4870.
[2] Wong S., Woolley A., Joselevich E., Cheung C., Lieber C. J., Am. Chem. Soc. 120, 1998, 8557.

Acknowledgements:
The authors thank B. Kalska-Szostko (Institute of Chemistry, University of Bialystok, Bialystok, Poland) for AFM measurements. We gratefully acknowledge for financial support from the State Committee for Scientific Research, Poland, projects: N N204 111535 to M.E.P.-B. and N N204 374733 to K.W.; the National Science Foundation, USA, Grant: DMR-0809129 to L.E. This material is based on work supported by the National Science Foundation while L.E. was working at the foundation. All opinions, findings, conclusions, or recommendations expressed herein are those of the authors and do not necessarily reflect the views of the National Science Foundation.

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This poster was presented on NanoBioView, 6-7 October 2010
http://www.nanobioviews.net

Authors:
Joanna Breczko,^a Magdalena Kierkowicz,^a Marta Eliza Plonska-Brzezinska,^a* Alina Dubis,^a Krzysztof Winkler ^a and Luis Echegoyen^b**

^a Institute of Chemistry, University of Bialystok, Hurtowa 1, 15-399 Bialystok, Poland, mplonska@uwb.edu.pl
^b Department of Chemistry, University of Texas at El Paso, 500W.

University Ave., El Paso, TX 79968, USA, echegoyen@utep.edu

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Keywords: AFM Atomic force microscopy Carbon Nano-Onions Fourier Transform Infrared Spectroscopy Luis Echegoyen Marta Eliza Plonska-Brzezinska Poster surface plasmon resonance Total Attenuated Reflactance Mode

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