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cLSM

Airyscanning
Aug. 24, 2014

Airyscanning

Confocal laser scanning microscopes (CLMs) are renowned for their sectioning capability. This feature is enabled by the use of pinhole, which rejects out-of-focus light. Less appreciated, on the other hand, is the gain in lateral resolution by this type of microscopes for one obvious reason. more
Body Architecture of the Parasitic Worm
Aug. 18, 2011

Body Architecture of the Parasitic Worm

Diplozoid monogenean Eudiplozoon nipponicum, with complex life cycle comprising oncomiracidium, diporpa, juvenile and adult stage, represents an ideal model for studies on morphological adaptations of metazoan parasites to the ectoparasitic life style. Combined morphological approach offers the advantage of more complex characterization and visualization of structures noticeable only when using specific microscopic method.

Crucial Questions to be Answered when Performing Scientific Research
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Optical Metrology Made Easy
Nov. 03, 2009

Optical Metrology Made Easy

Surface metrology is quickly emerging as a critical analytical technique to determine the topology of various materials. It can be used to identify corrosion, surface characterization, or to control the quality of different surfaces. Conventional methods such as profilometry, have involved the use of a stylus being dragged along the sample surface. However, this technique can be problematic; it cannot be used on certain materials, such as adhesives, and the dragging process itself may result in inaccurate data being obtained. more
Investigating the Material World
Nov. 02, 2009

Investigating the Material World

Corrosion is a big problem. From metal and concrete to polymers and electronics, different forms of decay lead to structural and functional failures that can sometimes be of fundamental importance. The Swedish Corrosion and Metals Institute - Swerea KIMAB, is focused on developing and improving solutions for materials research. As such, they are studying corrosion in its many forms as well as the various methods used to prevent it. more
Deep in vivo Imaging
Oct. 11, 2009

Deep in vivo Imaging

Based on the unique Olympus FluoView FV1000 confocal Laser Scanning Microscope (cLSM), the MPE system is available in three models providing the highest penetration depths on the market. The systems are fully configured to ensure best excitation efficiency and thus imaging hundreds of microns into a specimen is possible for the first time. more
Taking cLSM to Another Level
Oct. 11, 2009

Taking cLSM to Another Level

Confocal laser scanning microscopy provided scientists with the tools to see a much greater level of detail in cells. The Olympus FV1000 has taken this to an even higher level, with the added functionality and flexibility of the multi-laser combiner and SIM scanner. more
 A Spectral Revolution
Oct. 11, 2009

A Spectral Revolution

By removing out-of-focus light from above and below the focal plane, particularly in specimens >20 µm thick, the Olympus Disk Scanning Unit (DSU) uniquely delivers confocal-like images with excellent contrast and resolution compared to conventional wide-field microscopy. To reject the out-of-focus light, the DSU places a spinning disk, with an alternating pattern of vertical and horizontal slits, in the confocal plane of the microscope. more
Bringing Live Cells  into Focus
Nov. 02, 2008

Bringing Live Cells into Focus

The confocal laser scanning microscope (CLSM) is a key biomedical research tool. With progressive improvements in scanning speeds, image quality and the number of available functions, researchers have made many major breakthroughs. However, there are still biological processes which challenge even the fastest and most flexible CLSM. In response to this, Nikon has launched the A1 Series, suitable for the most demanding biomedical applications.

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Correlative 3D Microscopy: CLSM & FIB/SEM Tomography
Sep. 01, 2008

Correlative 3D Microscopy: CLSM & FIB/SEM Tomography

Correlative 3D Microscopy: CLSM & FIB/SEM Tomography -  A Study of Cellular Entry of Vaccinia Virus. Subcellular structural investigation on single cells or tissue samples requires the coupling of optimal structural preservation with detailed imaging at the light and electron microscopic level. more
Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data
Nov. 01, 2007

Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data

Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data. Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients. more
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