Dec. 12, 2012

Interdisciplinary Nanoscience Center Uses AFM Force Spectroscopy to Work at the Interface Between ...

The group of Dr Rikke Meyer from the interdisciplinary Nanoscience Center (iNANO) at Aarhus University, Denmark has used AFM and single-cell force spectroscopy to work at the interface between microbiology and nanoscience in the quest to understand how bacteria form biofilms and how this may be prevented. moreOct. 25, 2012

Visualizing Marine Microcosm with Confocal Laser Scanning Microscopy

The significance of small organic particles and organisms for the food webs of the oceans and for the global carbon cycle is generally known. However, many of the processes involved are not yet fully understood. moreJan. 11, 2012

Polarization Extension for Confocal Laser Scanning Microscopes Allows Fluorescence Anisotropy ...

Since several years, time-resolved fluorescence measurements can be performed with commercially available confocal laser scanning microscopes (LSM) that have been upgraded with a suited upgrade kit.  The polarization extension for PicoQuant's upgrade kit for LSM can now also perform polarization measurements. moreAug. 18, 2011

Body Architecture of the Parasitic Worm

Diplozoid monogenean Eudiplozoon nipponicum, with complex life cycle comprising oncomiracidium, diporpa, juvenile and adult stage, represents an ideal model for studies on morphological adaptations of metazoan parasites to the ectoparasitic life style. Combined morphological approach offers the advantage of more complex characterization and visualization of structures noticeable only when using specific microscopic method.

Crucial Questions to be Answered when Performing Scientific Research
moreJun. 10, 2011

Silicon Immersion Objectives for Long-Term or Deep-Penetration Imaging

Olympus has introduced UPLSAPO30xS and UPLSAPO60xS, two new silicon immersion objectives for life science imaging.
The lenses are ideal for live cell experiments, particularly those investigating thick samples or requiring long-term imaging. moreMay. 04, 2011

Intracellular Localisation of Fluorescently Labelled Nanoparticles

A laser scanning microscope collects information from a thin focal plane, disregarding out-of-focus information. It has become the standard imaging method to characterise cellular morphology and structures, both in static and living samples. Laser scanning microscopy at high resolution combined with digital image restoration is also a powerful tool to analyse intracellular localisation of fluorescently labelled nanoparticles (NPs), such as their colocalisation within membrane-bound compartments (e.g. moreOct. 11, 2009

A Spectral Revolution

By removing out-of-focus light from above and below the focal plane, particularly in specimens >20 µm thick, the Olympus Disk Scanning Unit (DSU) uniquely delivers confocal-like images with excellent contrast and resolution compared to conventional wide-field microscopy. To reject the out-of-focus light, the DSU places a spinning disk, with an alternating pattern of vertical and horizontal slits, in the confocal plane of the microscope. moreNov. 01, 2008

Systematic Analysis of FRAP Experiments

Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients

Introduction
moreMay. 31, 2006

ICN+T2006: Self-Assembling Dendrimers for Gene Transfection

ICN+T2006: Self-Assembling Dendrimers for Gene Transfection. One important goal within the first phase of the NCCR has been the design and synthesis of innovative DNA nano-carriers. The small amphiphilic cationic dendrimers have been indeed identified as promising transfection vectors, capable of delivering in vitro foreign DNA into the nucleus of cells [1]. moreMay. 31, 2005

Preparation protocol for multimodal imaging of biological tissue samples by light and electron ...

Preparation protocol for multimodal imaging of biological tissue samples by light and electron microscopy. A simple preparation protocol for multimodal imaging of biological tissue samples by light and electron microscopy.  more