Sep. 10, 2013
The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy.
moreAug. 22, 2013
The process cells use to ‘swallow' up nutrients, hormones and other signals from their environment - called endocytosis - can play a crucial role in shaping the cells themselves, scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, have found. The study, published in Nature Communications, could help explain how the cells on your skin become different from those that line your stomach or intestine.
moreAug. 01, 2013
A high-NA light microscope can be retro-fitted onto a Scanning Electron Microscope giving the possibility to perform simultaneous high-resolution fluorescence and electron microscopy on the same area of a sample.
moreJun. 19, 2013
It is a great pleasure to officially invite you to come and present your latest results at the Microscopy Conference 2013 in Regensburg, Germany. It will be held at the University of Regensburg, Germany, from August 25- 30, 2013. This conference follows the tradition of the "Dreiländertagung Mikroskopie".
moreJun. 18, 2013
Correlative light and electron microscopy (CLEM) combines the versatility of fluorescence microscopy (FLM) with the spatial resolution of transmission electron microscopy (TEM). For the analysis of tissues single resin or cryo-sections are incubated with fluorochrome- and gold-labeled probes. Areas of interest are selected at the FLM, and analyzed in the TEM at high resolution. This way, fluorescence is directly correlated to subcellular structures and/or corresponding immunogold signals.
moreApr. 16, 2013
We developed a new approach using cryo-correlative light and scanning transmission electron microscopy allowing analysis of targeted in situ intracellular ions and water measurements at the ultrastructural level within domains identified by examination of specific GFP-tagged proteins . We illustrate the potential of this approach by investigating changes in water and ion content in nuclear domains identified by GFP-tagged proteins in cells stressed by Actinomycin D treatment and controls.
moreJan. 23, 2013
Scandem 2013, the Annual Meeting of the Nordic Society for Microscopy, will be held in Copenhagen, Denmark, from the 12th to the 14th of June 2013. Pre-meeting workshops are organized on the 10th and the 11th of June. In addition to the regular Materials Science and Life Science talks using Electron Microscopy, there will be a focus on Correlative Microscopy and Super-Resolution Light Microscopy.
moreDec. 19, 2012
FEI Company has released a correlative microscopy solution set for light and electron microscopy (CLEM). By combining imaging techniques and efficient workflows, cell biologists could gain a better understanding of the structure and function of cells to improve the treatment of diseases, such as cancer, AIDS, and Parkinson's.
moreDec. 03, 2012
Scientists at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) and the BioEnergy Science Center (BESC) combined different microscopic imaging methods to gain a greater understanding of the relationships between biomass cell wall structure and enzyme digestibility, a breakthrough that could lead to optimizing sugar yields and lowering the costs of making biofuels. Results were published in Science.
moreAug. 29, 2012
We describe herein the development of a fast-scanning atomic force microscopy (AFM) combined with inverted fluorescent microscopy (FM), aiming at the elucidation of dynamic structure-function relationships of biological macromolecules on live cell membrane. Applicability of the system is demonstrated by the correlated AFM-FM imaging of chicken red blood cell (RBC) surfaces.