Nov. 01, 2008

Systematic Analysis of FRAP Experiments

Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients

Introduction
more