Nov. 24, 2011

Drag Reduction by DNA-grafting for Single Microspheres in a Dilute λ-DNA Solution

The fluid resistance of single micrometre-sized blank and DNA-grafted polystyrene microspheres under shear flow is compared in purified water and dilute λ-DNA solutions by means of optical tweezers experiments with a high spatial (±4 nm) and temporal (±0.2 ms) resolution.
moreJun. 20, 2011

Single-Molecule FRET & Super-Resolution

In order for nanotechnology, photonics and single-molecule spectroscopy to meet, structures with defined molecular compositions with dimensions in the 1-100 nm range are required. With the aid of DNA, nanostructures were constructed that guide light in switchable directions using multistep FRET from an input dye to an output dye. The direction of FRET is controlled by a jumper dye. moreJun. 10, 2011

Extended DNA Dynamics Experiments by Applying Atomic Force Microscopy –

JPK Instruments has reported on the work from the Pharmaceutical Sciences Department of the Medical School at the University of Nebraska. The Department has selected the JPK ForceRobot 300 system to extend their studies applying atomic force microscopy in the measurement of single molecule force spectroscopy. moreNov. 04, 2009

Catching Enzymes in the Act

Proteins that copy and edit DNA help protect cells from attack by removing viral DNA insertions. They are thought to reach their target sequence by "sliding", "hopping" and "jumping" along DNA strands. Observing this behavior has proved hard, however, due to its speed of action. We discuss a recent research project that used a high-speed camera to observe - for the first time - the sliding and jumping of EcoRV, a restriction enzyme, along DNA molecules.
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