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Super-Resolution Microscopy: Compressed Sensing Allows Imaging of Live Cell Structures
Despite many achievements in the field of super-resolution microscopy in the past few years with spatial resolution advances, live-cell imaging has remained a challenge because of the need for high temporal resolution. Researchers from the Georgia Institute of Technology and University of California San Francisco have advanced scientists' ability to view a clear picture of a single cellular structure in motion. more
New Ways to Super-Resolution: Reversible Chemical Reactions Control Fluorescent States
Recently, light microscopy has been revolutionized by novel approaches that circumvent the diffraction barrier the resolution limit of optical microscopes. Most of these novel methods are based on light-controlled switching of the labels fluorescent states and therefore require use of additional or more intense laser excitation lines. To overcome these demands a new probe has been developed which is controlled by a reversible chemical reaction thereby reducing the demands on the microscopes.Limitations of Light Microscopy
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Single-Molecule FRET & Super-Resolution
In order for nanotechnology, photonics and single-molecule spectroscopy to meet, structures with defined molecular compositions with dimensions in the 1-100 nm range are required. With the aid of DNA, nanostructures were constructed that guide light in switchable directions using multistep FRET from an input dye to an output dye. The direction of FRET is controlled by a jumper dye. more
Live-Cell Super-Resolution with dSTORM
Live-Cell Super-Resolution with dSTORM: A detailed microscopic characterization of cellular structures is important to understand cellular function. Conventional microscopy in some cases is limited by the achievable spatial resolution of about 200 nm in the imaging plane, which is not sufficient to reveal details at the near-molecular level. This is important if the organization of proteins in small organelles, clusters or machineries are studied. more
Super Resolution Microscopy: Light-independent Switching of Fluorescent Probes
Super Resolution Microscopy: a team of scientists headed by Dr. Dirk-Peter Herten at Heidelberg University's Institute of Physical Chemistry and members of the Cluster of Excellence "CellNetworks" have devised a new method in which light-dependent processes are replaced by chemical reactions to mark cellular structures for high-resolution optical microscopy. This method opens up new application vistas for fluorescence microscopy. more
dSTORM
Optical microscopes are subject to the diffraction barrier of light which imposes an optical resolution limit of approximately 200 nm in the imaging plane. dSTORM enables super-resolution imaging with commercially available standard fluorophores and probes at a resolution of ~ 20 nm even in living cells. more- Legal
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