Nov. 11, 2014
Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free imaging with restriction of signal generation to the focal spot of the scanning laser. It allows three-dimensional imaging of refraction index mismatches and of hemoglobin. We applied it to image mouse tissues and to characterize blood vessels and blood flow velocity. In small arteries, THG line scanning reveals the pulse velocity curve and hence the heart rate.
moreOct. 30, 2014
We review here a new generation of far-red fluorescent probes for live-cell imaging, which are based on a novel cell-permeable silicon rhodamine (SiR) dye. These probes combine a variety of desirable features, such as excellent selectivity, fluorogenicity, high brightness and low cytotoxicity, rendering them ideal probes for conventional and state-of-the-art super-resolution microscopy. Application of these new probes in combination with STED microscopy revealed for the first time the nine-fold symmetry of the centrosome and confirmed the spatial organization of actin in the axon of cultured neurons in living cells.
moreSep. 17, 2014
A system of compounds has been developed that can signal the presence of certain chemical compounds critical to cellular energy metabolism, as well as activating them. The work demonstrates an approach that the authors suggest could be used to control or investigate cells. They describe their approach as ‘bioparallel chemistry', emphasising the contrast with widely known bioorthogonal chemical reactions that do not interfere with biological processes native to the cell.
moreAug. 27, 2014
Angewandte Chemie publication: Picture a satellite orbiting the Earth and capturing numerous individual images of the planet at night in impressive definition, which, when combined to create one single large photograph, deliver an extremely detailed picture of life at night on the surface of the Earth. Only, in this case, the camera is not orbiting the Earth; it is traveling across human cells. And the light captured does not come from street lights, headlights, and lamps, but from specific structures inside the cell membrane or, more accurately, from glycoproteins and lipids illuminated using a special technique.
moreJun. 30, 2014
Robust sample preparation is key to any multiuser, high-end electron microscopy facility. At the EMBL our facility is permanently hosting 10 to 15 projects in parallel, and provides service to approximately 50 users per year. Each of these users face a number of different challenges when it comes to sample preparation and have different levels of experience. Our goal is to assist by standardizing methods and developing a toolbox to help projects become feasible and more efficient.
moreNov. 18, 2013
We propose a new durable multi-dimensional ruler for the calibration and the alignment of fluorescence microscopes (wide-field, confocal, multiphoton), thanks to non-photo-bleaching fluorescent sub-micrometer patterns embedded in glass in three dimensions. This new and robust tool enables the measurement of stage repositioning, detector's features, illumination homogeneity, both lateral and axial resolutions as well as the spectral shape, intensity and lifetime response of the system.
moreNov. 05, 2013
Craic Technologies has introduced the 508 PV UV-visible-NIR spectrophotometer for your microscope.
moreMay. 17, 2013
The group of Professor Markus Arndt at the University of Vienna, Austria, has shown the diffraction of single, massive molecules at a grating in an unambiguous demonstration of the wave-particle duality of quantum physics.
moreFeb. 14, 2013
Molecular Devices has introduced SpectraMax i3 Multi-Mode Microplate Detection Platform.
The system is available as a stand-alone reader with the option for users to upgrade to additional applications and detection modes such as cellular imaging with the SpectraMax MiniMax Imaging Cytometer, and cartridges for Time-Resolved Fluorescence, Fluorescence Polarization, and AlphaScreen assays.
moreFeb. 20, 2012
Lambda Square Mapping and FLIM: Leica Microsystems has developed Lambda Square Mapping (λ2) and FLIM for the comprehensive and easy characterization of complex fluorescent sample.