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Fluorescence Microscopy
Focus On Microscopy 2012
After the successful FOM2011 conference held in Konstanz this year, Focus on Microscopy 2012 (FOM2012) will be held in Singapore. It will take place in the week before Easter from Sunday April 1 to Wednesday April 4, 2012 at the Suntec Singapore International Convention & Exhibition Centre in the heart of the city. It is sponsored by the National University of Singapore (NUS), the Nanyang Technological University (NTU), the Centre of Bioimaging Sciences (CBIS), and the Mechanobiology Institute (MBI). more
Transient Absorption: Label-free Imaging of Nanotubes in Cells
Researchers have demonstrated a new imaging tool for tracking structures called carbon nanotubes in living cells and the bloodstream, which could aid efforts to perfect their use in biomedical research and clinical medicine.more
Dual Wavelength Imaging – The Ultimate Solution
The ORCA-D2 CCD camera is designed around two ER-150 CCD devices. It can capture simultaneous dual wavelength or multiple focal plane images. Each CCD captures a field of view measuring 1280 (H) x 960 (V) pixels, and each CCD has independent exposure and gain settings to accommodate significantly different intensity levels between the two images as is often seen in FRET and ratio imaging applications. more
Multi Color Laser Light Source for Super Resolution Microscopy
The Agilent Monolithic Laser Combiner MLC 400, distributed by Laser 2000 Europe, is a perfect solution for confocal, super resolution and fluorescence microscopy. Long-life DPSS and diode lasers are efficiently combined in a single output fiber, which is coupled to the microscope. With the integrated AOTF fast switching between the wavelengths and fast regulation of the output power is possible.The unique design includes permanent factory alignment. No more losing time with adjusting lasers instead of taking microscope images. more
Andor Introduces Differential Spinning Disk Uni
Andor Technology has introduced the Differential Spinning Disk (DSD) Uni. The DSD Uni brings with it the ability to add a confocal unit to almost any fluorescence microscope, upright or inverted, irrespective of manufacturer. more
Neo sCMOS Camera - Breakthrough Scientific CMOS Technology
The Neo sCMOS camera platform from Andor Technology stands alone in its ability to simultaneously offer ultra-low noise, extremely fast frame rates, wide dynamic range, high resolution and a large field of view, overcoming the performance trade-offs associated with traditional scientific CCD detectors. more
Synthetic Biology - A New Way to Access Biological Phenomena
The history of cell and molecular biology displays an arms race between biological challenges and their technological solutions, regarding the detection and analysis of ever smaller structures and processes. For a long time, advancements in microscopy have kept pace with scientific motivations. But since fundamental physical laws cannot be simply overcome, appropriate instruments need to become more and more complex if modern biology does not simply want to stand still due to technical limitations. more
Measuring Nanodistances in Cells
Understanding how complex processes such as receptor signal transduction work in cells requires knowledge of the structure-function relationships underlying the composition of protein complexes. Characterization of the oligomerization state of complexes requires the measurement of distances around 10-15 nm, too long for fluorescence energy transfer (FRET), but too small for optical resolution. Here we discuss various approaches that are being taken to measure these distances in cells.Spatial Resolution Requirements for Cell Biology
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Body Architecture of the Parasitic Worm
Diplozoid monogenean Eudiplozoon nipponicum, with complex life cycle comprising oncomiracidium, diporpa, juvenile and adult stage, represents an ideal model for studies on morphological adaptations of metazoan parasites to the ectoparasitic life style. Combined morphological approach offers the advantage of more complex characterization and visualization of structures noticeable only when using specific microscopic method.Crucial Questions to be Answered when Performing Scientific Research
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New Ways to Super-Resolution: Reversible Chemical Reactions Control Fluorescent States
Recently, light microscopy has been revolutionized by novel approaches that circumvent the diffraction barrier the resolution limit of optical microscopes. Most of these novel methods are based on light-controlled switching of the labels fluorescent states and therefore require use of additional or more intense laser excitation lines. To overcome these demands a new probe has been developed which is controlled by a reversible chemical reaction thereby reducing the demands on the microscopes.Limitations of Light Microscopy
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