Aug. 18, 2011

Quantitative Low-Light Fluorescence Imaging with Rolera Thunder EMCCD Camera from QImaging

QImaging has introduced the Rolera Thunder EMCCD microscopy camera, a high-sensitive, low-light fluorescence imaging EMCCD camera. Application Fields
• Super resolution microscopy (STORM, PALM)
• Single molecule fluorescence microscopy
• FRAP
• Astronomy
• Bose-Einstein condensate moreNov. 01, 2008

Systematic Analysis of FRAP Experiments

Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients

Introduction
moreNov. 01, 2007

Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data

Systematic Analysis of FRAP Experiments: Evaluation of Spatially Resolved Data. Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to study dynamic phenomena. Performing FRAP on a confocal laser scanning microscope documents the recovery process with high spatial resolution. This enables a consistent determination of the diffusion coefficient and the dimensionality of diffusion in calibration-free manner. Moreover, experiments representing multi-component diffusion can be analyzed as well, thus yielding the distribution of diffusion coefficients. moreNov. 01, 2007

FRET, FRAP, FISH - Technology & Techniques

FRET, FRAP, FISH - Technology & Techniques. The use of fluorescence microscopy in the life sciences runs the gamut from basic photodocumentation to dynamic single-molecule fluorescence (SMF) studies. Recent advances in digital imaging technology are helping to expand the utility and popularity of many fluorescence microscopy techniques, including Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), and fluorescence in situ hybridization (FISH). moreJan. 01, 2007

Advanced Optical Microscopy Course 2007

Advanced Optical Microscopy Course 2007: Biophysics is a molecular science rapidly moving to the nanoscale. It seeks to explain biological function in terms of the molecular structures and properties of specific molecules. The size of these molecules varies dramatically, from small fatty acids and sugars (~1 nm = 10-9 m), to macromolecules like proteins (5-10 nm), starches (bigger than 1,000 nm), and the enormously elongated DNA molecules. moreMay. 31, 2005

Fluorescence microscopy: biological applications and imaging methods

Fluorescence microscopy: biological applications and imaging methods - An Introduction more