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Single GFP-expressing Cell Is Basis of Living Laser Device
It sounds like something out of a comic book or a science fiction movie - a living laser - but that is exactly what two investigators at the Wellman Center for Photomedicine at Massachusetts General Hospital have developed. Wellman researchers Malte Gather, PhD, and Seok Hyun Yun, PhD, describe how a single cell genetically engineered to express green fluorescent protein (GFP) can be used to amplify the light particles called photons into nanosecond-long pulses of laser light. more
Electron Microscopy: Illuminating Life in Never-before-seen Detail
Led by Nobel laureate Roger Tsien , PhD, Howard Hughes Medical Institute investigator and UCSD professor of pharmacology, chemistry and biochemistry, a team of scientists radically re-engineered a light-absorbing protein from the cress plant Arabidopsis thaliana. When exposed to blue light, the altered protein produces abundant singlet oxygen, a form of molecular oxygen that can be made visible by electron microscopy (EM). more
Synthetic Biology: New Kind of Chimeric Fluorescent Biomolecule
A new kind of chimeric fluorescent biomolecule by means of synthetic biology was created by scientists from Technische Universitaet Muenchen (TUM) in Weihenstephan, Germany. They have managed to incorporate a synthetic amino acid into the natural green fluorescent protein (GFP). By exploiting a special physical effect, the fluorescent protein glows in turquoise when excited with ultraviolet light and displays up to now unmatched properties. more
Virimaging
It sounds like an idea plucked from the realms of science fiction writing. But in this case, there is nothing fictional about it. Scientists in Yorkshire have developed a process that uses the luminous cells from jellyfish to diagnose cancers deep within the human body. The method has been developed at the Yorkshire Cancer Research Laboratory at The University of York and the man who leads the York team, Professor Norman Maitland, believes it will revolutionize the way some cancers are diagnosed. more
Turn Up Brightness on Fluorescent Probes
Researchers from Carnegie Mellon University's Molecular Biosensor and Imaging Center (MBIC), USA are turning up the brightness on a group of fluorescent probes called fluoromodules that are used to monitor biological activities of individual proteins in real-time. This latest advance enhances their fluormodule technology by causing it to glow an order of magnitude brighter than typical fluorescent proteins. The new fluoromodules are five- to seven-times brighter than enhanced green fluorescent protein (EGFP), a development that will open new avenues for research. more
Protein with a Double Light Switch
The green fluorescent protein (GFP) of a pacific jellyfish was the first and, for a long time, the only one known. In recent years, researchers have discovered fluorescent proteins in other invertebrate marine fauna and also identified photactivatable proteins, the fluorescence of which can be controlled specifically by light irradiation. more
Giving Proteins a New Glow
Since the 1990s, a green fluorescent protein known simply as GFP has revolutionized cell biology. Originally found in a Pacific Northwest jellyfish, GFP allows scientists to visualize proteins inside of cells and track them as they go about their business. Two years ago, biologists who discovered and developed the protein as a laboratory tool won a Nobel Prize for their work. more
Molecular Structure of Key Fluorescent Proteins
Scientists at Albert Einstein College of Medicine of Yeshiva University, USA have determined the crystal structures of two key fluorescent proteins - one blue, one red - used to "light up" molecules in cells. That finding has allowed them to propose a chemical mechanism by which the red color in fluorescent proteins is formed from blue. With this information, the researchers now have the first roadmap for rationally designing new and differently colored fluorescent proteins to illuminate the structures and processes in living cells. more
Ca2+ Imaging
Effective fluorescence imaging of Ca2+ signals requires special filters for excitation of Fura 2 in UV at 340 nm and 380 nm. New hardcoated exciters with 95% transmission for ratio measurements are now available from AHF analysentechnik. Also emission filters with more than 95% transmission will enable maximum of signal intensity. Combining of GFP excitation and Fura 2 imaging can be done as well by using special filter set-ups. These filters can be mounted in all the different cubes of microscopes. moreJun. 01, 2008Fluorescence Lifetime Imaging: Refractive Index Sensing of GFP
Fluorescence Lifetime Imaging: Refractive Index Sensing of GFP - H.J. van Manen and his co-workers demonstrate the possibility to measure the local refractive index of intracellular GFPs by FLIM. Cytosolic and membrane-bound GFP fusion proteins showed a difference in fluorescence lifetimes that after comparison to a calibration curve could be used to determine the local refractive indices of cytosolic and membrane-targeted GFPs. This could be used to detect spatial heterogeneities within membraneous organelles. Biophys. J. 94, L67-L69 more- Legal
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