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Imaging

Correlative Light and Electron Microscopy
Jun. 30, 2014

Correlative Light and Electron Microscopy

Robust sample preparation is key to any multiuser, high-end electron microscopy facility. At the EMBL our facility is permanently hosting 10 to 15 projects in parallel, and provides service to approximately 50 users per year. Each of these users face a number of different challenges when it comes to sample preparation and have different levels of experience. Our goal is to assist by standardizing methods and developing a toolbox to help projects become feasible and more efficient.
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Jun. 26, 2014

CLARITY: Seeing the Inner Workings of the Brain

Bio-X scientists have improved on their original technique for peering into the intact brain, making it more reliable and safer. The results could help scientists unravel the inner connections of how thoughts, memories or diseases arise. more
What Is Hidden in the Volume Plasmon?
Jun. 10, 2014

What Is Hidden in the Volume Plasmon?

The volume plasmon excitation of valence electrons within soft organic matter contains a variety of characteristics responsible for their intrinsic physical and chemical properties. Up to now, the analysis of those properties (hardness, surface related signal) within Transmission Electron Microscopy (TEM) remains problematic due to the lack of methodological approaches which enable the extraction of required information from the ATEM data. Here we propose an empiric EFTEM based method that allows one to identify the latter.
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A Clearer Picture of Brain Circuitry with Single-Cell Resolution
May. 28, 2014

A Clearer Picture of Brain Circuitry with Single-Cell Resolution

An efficient method for whole-brain imaging at high-resolution makes it possible to visualize gene expression profiles, circuits and fine neuronal structures throughout the brain. Imaging of an entire brain at the resolution of single cells could lead to a deeper understanding of gene activation patterns and neuronal circuitry during development and disease. Existing whole-brain imaging methods, however, are equipment-, time- and labor-intensive, or tend to destroy the fluorescent labels used to visualize neurons.
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Apr. 01, 2014

Andor Management Change

Andor Technology, an Oxford Instruments company, offering scientific imaging and spectroscopy solutions announces the appointment of Gary Wilmot as Managing Director. Gary replaces Conor Walsh, who will be stepping down from the role, effective 1st May.
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Thermal Imaging of Bacterial Cells
Mar. 14, 2014

Thermal Imaging of Bacterial Cells

Since its invention Atomic Force Microscopy has established itself as a versatile tool for imaging material systems & associated dynamic processes. In the early 90s the technique was further developed to incorporate the use of heated probes as means of scanning the sample surface. In this study E.coli bacterial cells were used in an attempt to not only further develop and optimize the use of thermal AFM, but also to try and gain further insight into their interactions with pharmaceutical materials. more
Feb. 13, 2014

Andor Technology Joins Oxford Instruments

Oxford Instruments, a provider of high-technology tools for industry and research has recently acquired Andor. Andor is a  supplier of high performance cameras, microscope systems and software for the physical science and life science industries. Andor will continue to focus on growing their existing core markets and will spearhead Oxford Instruments strategic expansion into the Nano-Bio arena.
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Simple Processing in Fiji
Dec. 27, 2013

Simple Processing in Fiji

Microscopy has become an important element of the toolkit available to biologists. Advances in equipment and techniques have moved the microscope from a sole observational tool to a quantitative instrument. However, the requirements in terms of imaging in view of quantification, and image analysis may often seem daunting for non-specialists. In this communication, we propose a couple of simple and intuitive image analysis methodologies, evolving around median filtering, using functions in-built in Fiji [1]. The first application was to count labeled cells in mice tissue slices, which could be generalized to "object" counting in inhomogeneous samples. The second application was to quantify the signal to noise ratio (SNR) for each image of a z-stack, so that imaging parameters could be adapted to retain a constant SNR throughout the stack of images. more
What You Should Know about Image Noise
Dec. 09, 2013

What You Should Know about Image Noise

Insufficient light causes a snowstorm effect on many photos and videos, as a result of noise. It is important to know the different reasons for this phenomenon. The choice of the right camera for a particular application can help to eliminate the undesirable effect or to put it to good use. Pixel count, refresh rate and sensitivity are the decisive criteria for photographers when purchasing a camera. But they are also of interest to life scientists and users of microscopes. Unfortunately, there is another criterion that is often overlooked: the noise behavior of the camera or, to be more precise: its signal-to-noise ratio (SNR). Especially in professional applications, SNR is a major factor affecting image quality. Also, it is the characteristic that causes the greatest amount of misunderstanding. more
Characterization of Geological Samples
Dec. 02, 2013

Characterization of Geological Samples

Comprehensive investigations of geological samples can be challenging due to variations in size, shape, structure and composition. For that reason flexible analysis techniques are required which can be easily adapted to individual sample characteristics. more
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