Sep. 19, 2014
A record-setting X-ray microscopy experiment may have ushered in a new era for nanoscale imaging. Working at the U.S. Department of Energy (DOE)´s Lawrence Berkeley National Laboratory (Berkeley Lab), a collaboration of researchers used low energy or "soft" X-rays to image structures only five nanometres in size. This resolution, obtained at Berkeley Lab´s Advanced Light Source (ALS), a DOE Office of Science User Facility, is the highest ever achieved with X-ray microscopy.
moreAug. 27, 2014
Neurotransmitters play an important role in the communication of nerve cells. Major details of the processes involved have been unclear until recently. Scientists of the University of Würzburg have now shed light on these processes by using a new technique.
moreAug. 25, 2014
Laser physicists have found a way to make atomic-force microscope probes 20 times more sensitive and capable of detecting forces as small as the weight of an individual virus.
The technique, developed by researchers at The Australian National University (ANU), hinges on using laser beams to cool a nanowire probe to minus 265 degrees Celsius.
moreAug. 24, 2014
Confocal laser scanning microscopes (CLMs) are renowned for their sectioning capability. This feature is enabled by the use of pinhole, which rejects out-of-focus light. Less appreciated, on the other hand, is the gain in lateral resolution by this type of microscopes for one obvious reason.
moreAug. 20, 2014
COIN: Göttingen Scientists develop a combined technique for studying cellular structures via high-resolution imaging.
moreAug. 07, 2014
In the year 2008 Nature Methods has selected super-resolution microscopy or nanoscopy as "method of the year" . Since that time super-resolution microscopy has emerged from specialized physics laboratories and become a powerful tool for biologists. The field of application is constantly growing and since recently even expanding in the axial dimension for single molecule localization microscopy. The process to get high quality super-resolution images can be divided in three important steps: sample preparation, image acquisition and image processing. Especially the first step has to be considered with a lot of care, since higher resolving power demands more stringent sample preparation.
moreJun. 30, 2014
Robust sample preparation is key to any multiuser, high-end electron microscopy facility. At the EMBL our facility is permanently hosting 10 to 15 projects in parallel, and provides service to approximately 50 users per year. Each of these users face a number of different challenges when it comes to sample preparation and have different levels of experience. Our goal is to assist by standardizing methods and developing a toolbox to help projects become feasible and more efficient.
moreJun. 26, 2014
Bio-X scientists have improved on their original technique for peering into the intact brain, making it more reliable and safer. The results could help scientists unravel the inner connections of how thoughts, memories or diseases arise.
moreJun. 10, 2014
The volume plasmon excitation of valence electrons within soft organic matter contains a variety of characteristics responsible for their intrinsic physical and chemical properties. Up to now, the analysis of those properties (hardness, surface related signal) within Transmission Electron Microscopy (TEM) remains problematic due to the lack of methodological approaches which enable the extraction of required information from the ATEM data. Here we propose an empiric EFTEM based method that allows one to identify the latter.
moreMay. 28, 2014
An efficient method for whole-brain imaging at high-resolution makes it possible to visualize gene expression profiles, circuits and fine neuronal structures throughout the brain. Imaging of an entire brain at the resolution of single cells could lead to a deeper understanding of gene activation patterns and neuronal circuitry during development and disease. Existing whole-brain imaging methods, however, are equipment-, time- and labor-intensive, or tend to destroy the fluorescent labels used to visualize neurons.