Dec. 09, 2013
Insufficient light causes a snowstorm effect on many photos and videos, as a result of noise. It is important to know the different reasons for this phenomenon. The choice of the right camera for a particular application can help to eliminate the undesirable effect or to put it to good use. Pixel count, refresh rate and sensitivity are the decisive criteria for photographers when purchasing a camera. But they are also of interest to life scientists and users of microscopes. Unfortunately, there is another criterion that is often overlooked: the noise behavior of the camera or, to be more precise: its signal-to-noise ratio (SNR). Especially in professional applications, SNR is a major factor affecting image quality. Also, it is the characteristic that causes the greatest amount of misunderstanding.
moreDec. 02, 2013
Comprehensive investigations of geological samples can be challenging due to variations in size, shape, structure and composition. For that reason flexible analysis techniques are required which can be easily adapted to individual sample characteristics.
moreOct. 31, 2013
Which optical microscopy methods gain more and more attention at the moment? Which are the advantages and the main application? Statements from Jens Rietdorf, Director Carl Zeiss Microscopy Labs, during an interview with Imaging & Microscopy on Tour 2013.
moreSep. 10, 2013
The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy.
moreAug. 07, 2013
We combined holographic optical tweezers (HOT) with self-interference digital holographic microscopy (DHM). HOT allow flexible three-dimensional contactless moving of particles inside cells and positioning of bacteria on host cells while self-interference DHM provides simplified multi-focus quantitative phase imaging and 3D object tracking. Our results demonstrate novel ways for the analysis of the intracellular morphology and modeling of infection scenarios at single cell scale.
moreJul. 22, 2013
We explore the potential of a novel imaging method to exploit differential susceptibility of biological tissue to erosion by Focused Ion Beam (FIB) milling. The contents of a given cell (nucleus, Golgi apparatus, vacuoles) have different physical compositions and will ablate differentially under the ion beam. Sequential fine slices of frozen cells have been ablated, after which slice-by-slice image analysis of the extracted ablation vectors is back-converted to a FIB susceptibility parameter.
moreJul. 17, 2013
NKT´s SuperK Extend-UV wavelength extension unit provides tunable light in the entire 270-400nm range with power levels on the order of 10-100 μW.
moreJul. 12, 2013
Available on the iXon Ultra 897 camera, the Optically Centred Crop Mode offers extremely fast frame rate performance from Region of Interest (ROI), and is ideally matched to the speed and sensitivity needs of live cell super-resolution microscopy.
moreJun. 08, 2013
The symposium "Seeing is Believing - Imaging the Processes of Life" will take place at the EMBL Advanced Training Centre, Heidelberg, Germany from October 3 - 6, 2013. With the symposium we aim to bring together the leading developers of imaging methods with cutting edge applications that illustrate how imaging can answer biological questions. We will place emphasis on methods that are able to capture the dynamics of life and aim to span the whole range from molecular resolution to imaging of whole organisms.
moreMay. 13, 2013
Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically - which was associated with the term "magic bullet", the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis - though not specific enough not to cause substantial side effects.