Mar. 14, 2014
Since its invention Atomic Force Microscopy has established itself as a versatile tool for imaging material systems & associated dynamic processes. In the early 90s the technique was further developed to incorporate the use of heated probes as means of scanning the sample surface. In this study E.coli bacterial cells were used in an attempt to not only further develop and optimize the use of thermal AFM, but also to try and gain further insight into their interactions with pharmaceutical materials.
moreFeb. 13, 2014
Oxford Instruments, a provider of high-technology tools for industry and research has recently acquired Andor. Andor is a supplier of high performance cameras, microscope systems and software for the physical science and life science industries. Andor will continue to focus on growing their existing core markets and will spearhead Oxford Instruments strategic expansion into the Nano-Bio arena.
moreDec. 27, 2013
Microscopy has become an important element of the toolkit available to biologists. Advances in equipment and techniques have moved the microscope from a sole observational tool to a quantitative instrument. However, the requirements in terms of imaging in view of quantification, and image analysis may often seem daunting for non-specialists. In this communication, we propose a couple of simple and intuitive image analysis methodologies, evolving around median filtering, using functions in-built in Fiji . The first application was to count labeled cells in mice tissue slices, which could be generalized to "object" counting in inhomogeneous samples. The second application was to quantify the signal to noise ratio (SNR) for each image of a z-stack, so that imaging parameters could be adapted to retain a constant SNR throughout the stack of images.
moreDec. 09, 2013
Insufficient light causes a snowstorm effect on many photos and videos, as a result of noise. It is important to know the different reasons for this phenomenon. The choice of the right camera for a particular application can help to eliminate the undesirable effect or to put it to good use. Pixel count, refresh rate and sensitivity are the decisive criteria for photographers when purchasing a camera. But they are also of interest to life scientists and users of microscopes. Unfortunately, there is another criterion that is often overlooked: the noise behavior of the camera or, to be more precise: its signal-to-noise ratio (SNR). Especially in professional applications, SNR is a major factor affecting image quality. Also, it is the characteristic that causes the greatest amount of misunderstanding.
moreDec. 02, 2013
Comprehensive investigations of geological samples can be challenging due to variations in size, shape, structure and composition. For that reason flexible analysis techniques are required which can be easily adapted to individual sample characteristics.
moreOct. 31, 2013
Which optical microscopy methods gain more and more attention at the moment? Which are the advantages and the main application? Statements from Jens Rietdorf, Director Carl Zeiss Microscopy Labs, during an interview with Imaging & Microscopy on Tour 2013.
moreSep. 10, 2013
The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy.
moreAug. 07, 2013
We combined holographic optical tweezers (HOT) with self-interference digital holographic microscopy (DHM). HOT allow flexible three-dimensional contactless moving of particles inside cells and positioning of bacteria on host cells while self-interference DHM provides simplified multi-focus quantitative phase imaging and 3D object tracking. Our results demonstrate novel ways for the analysis of the intracellular morphology and modeling of infection scenarios at single cell scale.
moreJul. 22, 2013
We explore the potential of a novel imaging method to exploit differential susceptibility of biological tissue to erosion by Focused Ion Beam (FIB) milling. The contents of a given cell (nucleus, Golgi apparatus, vacuoles) have different physical compositions and will ablate differentially under the ion beam. Sequential fine slices of frozen cells have been ablated, after which slice-by-slice image analysis of the extracted ablation vectors is back-converted to a FIB susceptibility parameter.
moreJul. 17, 2013
NKT´s SuperK Extend-UV wavelength extension unit provides tunable light in the entire 270-400nm range with power levels on the order of 10-100 μW.