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Oct. 31, 2013

Optical Microscopy Methods (Video)

Which optical microscopy methods gain more and more attention at the moment? Which are the advantages and the main application? Statements from Jens Rietdorf, Director Carl Zeiss Microscopy Labs, during an interview with Imaging & Microscopy on Tour 2013. more
Multiscale and Multimodal Correlative Microscopy Workflows
Sep. 10, 2013

Multiscale and Multimodal Correlative Microscopy Workflows

The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy. more
Combined Holographic Optical Trapping and Imaging
Aug. 07, 2013

Combined Holographic Optical Trapping and Imaging

We combined holographic optical tweezers (HOT) with self-interference digital holographic microscopy (DHM). HOT allow flexible three-dimensional contactless moving of particles inside cells and positioning of bacteria on host cells while self-interference DHM provides simplified multi-focus quantitative phase imaging and 3D object tracking. Our results demonstrate novel ways for the analysis of the intracellular morphology and modeling of infection scenarios at single cell scale. more
Focused Ion Beam Ablation Tomography
Jul. 22, 2013

Focused Ion Beam Ablation Tomography

We explore the potential of a novel imaging method to exploit differential susceptibility of biological tissue to erosion by Focused Ion Beam (FIB) milling. The contents of a given cell (nucleus, Golgi apparatus, vacuoles) have different physical compositions and will ablate differentially under the ion beam. Sequential fine slices of frozen cells have been ablated, after which slice-by-slice image analysis of the extracted ablation vectors is back-converted to a FIB susceptibility parameter. more
Tunable Deep-UV Supercontinuum-powered Laser
Jul. 17, 2013

Tunable Deep-UV Supercontinuum-powered Laser

NKT´s SuperK Extend-UV wavelength extension unit provides tunable light in the entire 270-400nm range with power levels on the order of 10-100 μW. more
Andor Introduces Optically Centred Crop Mode for Live Cell Super-Resolution Microscopy
Jul. 12, 2013

Andor Introduces Optically Centred Crop Mode for Live Cell Super-Resolution Microscopy

Available on the iXon Ultra 897 camera, the Optically Centred Crop Mode offers extremely fast frame rate performance from Region of Interest (ROI), and is ideally matched to the speed and sensitivity needs of live cell super-resolution microscopy.  more
Seeing Is Believing – Imaging the Processes of Life
Jun. 08, 2013

Seeing Is Believing – Imaging the Processes of Life

The symposium "Seeing is Believing - Imaging the Processes of Life" will take place at the EMBL Advanced Training Centre, Heidelberg, Germany from October 3 - 6, 2013. With the symposium we aim to bring together the leading developers of imaging methods with cutting edge applications that illustrate how imaging can answer biological questions. We will place emphasis on methods that are able to capture the dynamics of life and aim to span the whole range from molecular resolution to imaging of whole organisms.
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Unmask the Hidden without Probes: CARS
May. 13, 2013

Unmask the Hidden without Probes: CARS

Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically - which was associated with the term "magic bullet", the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis - though not specific enough not to cause substantial side effects. more
Molecularly Resolved Cellulose Nitrate
Apr. 02, 2013

Molecularly Resolved Cellulose Nitrate

The development of nanostructured lacquers and propellants needs to master the cellulose nitrate processing on the nanoscale. The challenge is the deposition of single cellulose nitrate molecules to image them with molecular resolution. We report on the effect of solvent, shaking duration and deposition techniques, from which the spray technique succeeded, for the first time to our knowledge, to image by Atomic Force Microscopy, individual molecular cellulose nitrate polymeric chains.
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Nano-FTIR - The Chemical Nanoscope
Mar. 25, 2013

Nano-FTIR - The Chemical Nanoscope

We show identification of chemical compounds and chemical maps at 20 nm resolution, enabled by a novel combination of infrared spectroscopy and near-field microscopy. Nano-FTIR returns the surface topography and simultaneously the local mid-infrared spectrum of the tiny volume (20 nm3) just below the probing tip thus allowing correlative topography/hyperspectral infrared images. In the case of molecular substances, comparison with common infrared databases enables local chemical recognition.
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