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Light Microscopy

How to Set Up a 3D-SIM Microscope (User Manual)
Apr. 01, 2014

How to Set Up a 3D-SIM Microscope (User Manual)

In the past two decades, light microscopy has seen a tremendous improvement with super-resolution techniques. Many of the super-resolution microscopes (3D-SIM, STED, and PALM/STORM) are now available in a commercial solution, and are entering labs and facilities worldwide. This offers an important step forward in the field of research in biology, but when not used in optimal conditions, those powerful techniques can give rise to artefacts. Here we focus on parameters that can deeply influence the image quality for 3D-SIM and the next challenges for this technique. more
14th International ELMI Meeting
Jan. 06, 2014

14th International ELMI Meeting

The 14th European Light Microscopy Initiative (ELMI2014) conference will be held in Oslo, Norway from May 20-23, 2014. ELMI is an internationally recognized meeting on Light Microscopy and offers a unique combination of lectures, exhibitions and hands-on workshops organized by leading microscopy companies. The meeting is aimed at a life sciences audience and specifically focuses on close collaborative interactions between companies, developers and researchers. Imaging methods from a wide range of research fields will be presented that are relevant to both new and expert users of electronic and photonic methods.
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Why One Disk Is Better Than Two
Nov. 25, 2013

Why One Disk Is Better Than Two

A novel optical concept for a spinning disk confocal microscope is presented, which warrants maximal optical quality, speed and usability. It employs a single disk only instead of two, and it uses micromirrors instead of microlenses, thus minimizing chromatic aberrations and yielding an uncompromised performance over the full visible range from 405 - 700 nm. Careful optimization of coupling optics result in flat and homogenous illumination free of speckle artifacts, and the full optical performance is maintained over the full field of view. 
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Bioluminescence Microscopy
Nov. 12, 2013

Bioluminescence Microscopy

In contrast to fluorescence methods, bioluminescence microscopy does not need excitation by light. As photon emission results from a chemical reaction, results are highly specific and quantifiable. Until recently bioluminescence microscopy was difficult to approach as a result of rather dim signal intensities. Due to better probes and especially thanks to better and more specific instrumentation this technique has now become much more accessible and can in many situations outperform fluorescent approaches. more
3D Imaging of the Brain: Overcoming Some Limitations of Light Microscopy
Sep. 10, 2013

3D Imaging of the Brain: Overcoming Some Limitations of Light Microscopy

Scientists at the Campus Vienna Biocenter (Austria) have found a way to overcome some of the limitations of light microscopy. Applying the new technique, they can record the activity of a worm's brain with high temporal and spatial resolution, ultimately linking brain anatomy to brain function. The journal Nature Methods publishes the details in its current issue.
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Multiscale and Multimodal Correlative Microscopy Workflows
Sep. 10, 2013

Multiscale and Multimodal Correlative Microscopy Workflows

The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy. more
Aug. 22, 2013

Correlative Microscopy: Specialized Endocytocis Consumes Membrane Tendrils

The process cells use to ‘swallow' up nutrients, hormones and other signals from their environment - called endocytosis - can play a crucial role in shaping the cells themselves, scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, have found. The study, published in Nature Communications, could help explain how the cells on your skin become different from those that line your stomach or intestine.
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New Microscopy Lab for Light and Electron Microscopy at the Nencki Institute in Warsaw
Aug. 22, 2013

New Microscopy Lab for Light and Electron Microscopy at the Nencki Institute in Warsaw

A combination of devices for light and electron microscopy has been installed in the Neurobiology Centre of the Nencki Institute, Warsaw, Poland. This equipment will soon be applied by researchers to better understand the structure, function and capabilities of the human brain.
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Arizona State University: Plasmonic Resonance Microscopy System
Aug. 14, 2013

Arizona State University: Plasmonic Resonance Microscopy System

Peering through a homemade instrument-toy-like by today's standards-the Dutch tradesman Antony van Leeuwenhoek (1632-1723) first observed a dizzying menagerie of lifeforms, invisible to the naked eye. Since then, scientists have steadily refined the field of microscopy, achieving spectacular results at ever-tinier scales.
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Combined Holographic Optical Trapping and Imaging
Aug. 07, 2013

Combined Holographic Optical Trapping and Imaging

We combined holographic optical tweezers (HOT) with self-interference digital holographic microscopy (DHM). HOT allow flexible three-dimensional contactless moving of particles inside cells and positioning of bacteria on host cells while self-interference DHM provides simplified multi-focus quantitative phase imaging and 3D object tracking. Our results demonstrate novel ways for the analysis of the intracellular morphology and modeling of infection scenarios at single cell scale. more
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