Apr. 22, 2014
A high-speed scanning solution for transmitted-light microscopy which allows scanning of microtiter plates at unprecedented speed was developed at the Fraunhofer-Institute for Production Technology IPT in Aachen, Germany. The novel solution is based on a continuously moving scanning stage with synchronous z-position adjustment and flash illumination.
moreApr. 01, 2014
In the past two decades, light microscopy has seen a tremendous improvement with super-resolution techniques. Many of the super-resolution microscopes (3D-SIM, STED, and PALM/STORM) are now available in a commercial solution, and are entering labs and facilities worldwide. This offers an important step forward in the field of research in biology, but when not used in optimal conditions, those powerful techniques can give rise to artefacts. Here we focus on parameters that can deeply influence the image quality for 3D-SIM and the next challenges for this technique.
moreJan. 06, 2014
The 14th European Light Microscopy Initiative (ELMI2014) conference will be held in Oslo, Norway from May 20-23, 2014. ELMI is an internationally recognized meeting on Light Microscopy and offers a unique combination of lectures, exhibitions and hands-on workshops organized by leading microscopy companies. The meeting is aimed at a life sciences audience and specifically focuses on close collaborative interactions between companies, developers and researchers. Imaging methods from a wide range of research fields will be presented that are relevant to both new and expert users of electronic and photonic methods.
moreNov. 25, 2013
A novel optical concept for a spinning disk confocal microscope is presented, which warrants maximal optical quality, speed and usability. It employs a single disk only instead of two, and it uses micromirrors instead of microlenses, thus minimizing chromatic aberrations and yielding an uncompromised performance over the full visible range from 405 - 700 nm. Careful optimization of coupling optics result in flat and homogenous illumination free of speckle artifacts, and the full optical performance is maintained over the full field of view.
moreNov. 12, 2013
In contrast to fluorescence methods, bioluminescence microscopy does not need excitation by light. As photon emission results from a chemical reaction, results are highly specific and quantifiable. Until recently bioluminescence microscopy was difficult to approach as a result of rather dim signal intensities. Due to better probes and especially thanks to better and more specific instrumentation this technique has now become much more accessible and can in many situations outperform fluorescent approaches.
moreSep. 10, 2013
Scientists at the Campus Vienna Biocenter (Austria) have found a way to overcome some of the limitations of light microscopy. Applying the new technique, they can record the activity of a worm's brain with high temporal and spatial resolution, ultimately linking brain anatomy to brain function. The journal Nature Methods publishes the details in its current issue.
moreSep. 10, 2013
The ability to efficiently and reliably locate and image an area of interest in a larger three dimensional sample remains a very challenging application in advanced microscopy. Approaches to address this issue include dedicated sample preparation steps to cut out the target area, or optical penetration using dedicated confocal systems. Both approaches are labor intense and cannot be automated easily. With X-ray microscopy, a new technology has evolved that shows a huge potential to bridge the gap between light and electron microscopy.
moreAug. 22, 2013
The process cells use to ‘swallow' up nutrients, hormones and other signals from their environment - called endocytosis - can play a crucial role in shaping the cells themselves, scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, have found. The study, published in Nature Communications, could help explain how the cells on your skin become different from those that line your stomach or intestine.
moreAug. 22, 2013
A combination of devices for light and electron microscopy has been installed in the Neurobiology Centre of the Nencki Institute, Warsaw, Poland. This equipment will soon be applied by researchers to better understand the structure, function and capabilities of the human brain.
moreAug. 14, 2013
Peering through a homemade instrument-toy-like by today's standards-the Dutch tradesman Antony van Leeuwenhoek (1632-1723) first observed a dizzying menagerie of lifeforms, invisible to the naked eye. Since then, scientists have steadily refined the field of microscopy, achieving spectacular results at ever-tinier scales.