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live cell imaging

Apr. 09, 2014

Video: cellVivo-The Modular and Flexible Incubation System

Extended live cell imaging requires stringent control of temperature, humidity, CO2 and O2 levels for sample integrity and focus position. In this short video, Olympus introduces its cellVivo incubation system for precise and ergonomic environmental control of advanced live cell imaging. more
Membrane Protein Localization by SpecON
Apr. 08, 2014

Membrane Protein Localization by SpecON

Spectrally coded optical nano-sectioning (SpecON) is a high-resolution microscopy technique that translates spatial (position) information of fluorescent markers into spectral (color) information providing a protein localization precision of up to 5-10 nm in live cells. The key element is a thin metal-dielectric coating on a microscope slide. The biocompatible design is such that the distance-dependent spectral "fingerprint" of fluorophores can be used to monitor their relative distance from the coating to study the positions and dynamics of key proteins in cell motility. more
Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity
Apr. 02, 2014

Structured Illumination Microscopy: Chemical Probe Profiles Live-cell Organelle Activity

A team of scientists from Pacific Northwest National Laboratory synthesized a chemical activity-based probe (ABP) that can provide new information about how living cells function. The new ABP is designed to enter a living cell without interacting with anything until it enters a specific organelle: the lysosome. This proof-of-concept ABP then labels only functionally active enzymes called cathepsins, which are cysteine proteases, in the lysosome. Using proteomics and super-resolution microscopy to view these labeled enzymes, the scientists now are able to see organellar activity. Their work, which demonstrates the ability to manipulate chemistry to better understand biology, has been published in Angewandte Chemie International Edition.
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How to Set Up a 3D-SIM Microscope (User Manual)
Apr. 01, 2014

How to Set Up a 3D-SIM Microscope (User Manual)

In the past two decades, light microscopy has seen a tremendous improvement with super-resolution techniques. Many of the super-resolution microscopes (3D-SIM, STED, and PALM/STORM) are now available in a commercial solution, and are entering labs and facilities worldwide. This offers an important step forward in the field of research in biology, but when not used in optimal conditions, those powerful techniques can give rise to artefacts. Here we focus on parameters that can deeply influence the image quality for 3D-SIM and the next challenges for this technique. more
4D Live Imaging of the Cellular Shipping Port
Mar. 26, 2014

4D Live Imaging of the Cellular Shipping Port

The epithelium lines the organs of the human body. In the skin and the intestine as well as in the kidney, this cell layer forms a barrier that regulates the exchange of molecules like hormones and nutrients. The Freiburg biophysicist Dr. Roland Thünauer and junior professor Dr. Winfried Römer, Institute of Biology II and member of the Cluster of Excellence BIOSS Centre for Biological Signalling Studies of the University of Freiburg, have discovered a new mechanism by which proteins are transported to the outer membrane in epithelial cells.
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White-Light Diffraction Tomography: Looking into Cells without Using Dyes
Jan. 29, 2014

White-Light Diffraction Tomography: Looking into Cells without Using Dyes

Living cells are ready for their close-ups, thanks to a new imaging technique that needs no dyes or other chemicals, yet renders high-resolution, three-dimensional, quantitative imagery of cells and their internal structures - all with conventional microscopes and white light. Called white-light diffraction tomography (WDT), the imaging technique opens a window into the life of a cell without disturbing it and could allow cellular biologists unprecedented insight into cellular processes, drug effects and stem cell differentiation. The team of University of Illinois researchers, led by electrical and computer engineering and bioengineering professor Gabriel Popescu, published their results in Nature Photonics.
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Medical Imaging: Breakthrough in Fiber-optical Two-photon Fluorescence Endoscopy
Jan. 25, 2014

Medical Imaging: Breakthrough in Fiber-optical Two-photon Fluorescence Endoscopy

A breakthrough technique for super-resolution 3D medical imaging of living cells has been developed by researchers at Swinburne University of Technology. The new technique potentially could aid in minimally-invasive surgery and the early detection of cancer. The research is published in Scientific Reports.
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Live Cell Nanoscopy: Studying the Motion of Cells with High Spacial Resolution
Jan. 17, 2014

Live Cell Nanoscopy: Studying the Motion of Cells with High Spacial Resolution

Scientists from the University of Göttingen have developed a new technique which allows for observing the motion of living cells with a resolution that hadn't been possible before. The new optical method enables scientists to trace the motion of individual cells with the spatial resolution of a millionth part of a millimeter in real time. Until now, the changes in a living cell's shape could only be measured with the accuracy of a little better than a thousandth part of a millimeter. The details were published in the journal Nature Photonics.
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14th International ELMI Meeting
Jan. 06, 2014

14th International ELMI Meeting

The 14th European Light Microscopy Initiative (ELMI2014) conference will be held in Oslo, Norway from May 20-23, 2014. ELMI is an internationally recognized meeting on Light Microscopy and offers a unique combination of lectures, exhibitions and hands-on workshops organized by leading microscopy companies. The meeting is aimed at a life sciences audience and specifically focuses on close collaborative interactions between companies, developers and researchers. Imaging methods from a wide range of research fields will be presented that are relevant to both new and expert users of electronic and photonic methods.
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Nov. 28, 2013

Bioaxial Secures € 1.9 Million in New Funding

Bioaxial, developers of super-resolution fluorescence microscopy for the extended imaging of live cells, announces the completion of a € 1.9 million equity investment by three new large investors; Amorcage Technologique Investissement, Inserm Transfert Initiative and Viveris Management plus a range of individual investors.
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