Apr. 04, 2011

Live-Cell Super-Resolution with dSTORM

Live-Cell Super-Resolution with dSTORM: A detailed microscopic characterization of cellular structures is important to understand cellular function. Conventional microscopy in some cases is limited by the achievable spatial resolution of about 200 nm in the imaging plane, which is not sufficient to reveal details at the near-molecular level. This is important if the organization of proteins in small organelles, clusters or machineries are studied. moreAug. 12, 2010

Living Cells Made to Fluoresce

Individual molecules and their dynamics can also be made visible in living cells using conventional fluorophores at a resolution of around 20 nanometers. How this is done is being revealed for the first time by researchers from Würzburg, Bielefeld, and New York in the journal Nature Methods. moreMay. 03, 2010

dSTORM

Optical microscopes are subject to the diffraction barrier of light which imposes an optical resolution limit of approximately 200 nm in the imaging plane. dSTORM enables super-resolution imaging with commercially available standard fluorophores and probes at a resolution of ~ 20 nm even in living cells. more